Protein Info for Pf6N2E2_960 in Pseudomonas fluorescens FW300-N2E2

Annotation: Type IV secretory pathway, VirD4 components

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 706 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 46 to 63 (18 residues), see Phobius details TIGR03754: conjugative coupling factor TraD, PFGI-1 class" amino acids 10 to 658 (649 residues), 1117.6 bits, see alignment E=0 TIGR03743: conjugative coupling factor TraD, SXT/TOL subfamily" amino acids 10 to 658 (649 residues), 882.5 bits, see alignment E=1.7e-269 PF12696: TraG-D_C" amino acids 508 to 638 (131 residues), 48.1 bits, see alignment E=1.2e-16

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfo:Pfl01_3012)

Predicted SEED Role

"Type IV secretory pathway, VirD4 components"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZSD7 at UniProt or InterPro

Protein Sequence (706 amino acids)

>Pf6N2E2_960 Type IV secretory pathway, VirD4 components (Pseudomonas fluorescens FW300-N2E2)
VSQVMAEHAMESKLRPAVELYTVAICIAVAVLCVYSPWAVALSPEIGLAAALAYTLFGLI
RLRQAWEVLRYRRNIRRLPRYELTSKQIPVSRTRLFMGLGFRWTRLHTQRLVEAQDPAVA
HYVDQPTSYSLARELERRLEHAPFPLSTLARVTAWDSAFNPLRPLPPVGGSPLLHGVEPN
ETEVSLPLGERVGHTLVLGTTRVGKTRLAEVYITQDIHRVEHEVVIVFDPKGDADLLKRM
YVEAKRAGREKEFYVFHLGWPEISARYNAVGRFGRISEVASRIAGQLSGEGNSAAFREFA
WRFVNIIARALIELGRRPDYLQIQRHVVNIDALFIEYAQQFFAKTDPKAWEVIVQLESKL
TEKNIPRHMVGREKRVVAIEQYLAAKRVFDPVMDGLRSAVRYDRTYFDKIVASLLPLLEK
LTSGKTAQLLAPNYTDLDDPRPIFDWMQIIRKRGIVYVGLDALTDAEVAAAVGNSMFADL
VSVAGHIYKHGIDHGLPQSGNNSSAKLPINLHADEFNELMGDEFIPLINKGGGAGIQVTA
YTQTLSDIEARIGNRAKAGQVIGNFNTLQMLRVRETATAELLTQQLPKVNVLTKTLVSGA
TDTSDPEANTDFTSSSQDRVSSTSVPLIEPAHIISLPKGQMFSFQAGGQLWKVRMPLPKP
SNDDAMPKDLQELTQRMRAAYNAQAGQWWSASGGGPTTNFDLDQVG