Protein Info for Pf6N2E2_925 in Pseudomonas fluorescens FW300-N2E2
Annotation: Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 92% identity to pfo:Pfl01_2769)Predicted SEED Role
"Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140)" in subsystem D-Sorbitol(D-Glucitol) and L-Sorbose Utilization (EC 1.1.1.140)
MetaCyc Pathways
- hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
- D-sorbitol degradation II (1/1 steps found)
- L-sorbose degradation (1/2 steps found)
- superpathway of hexitol degradation (bacteria) (12/18 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.140
Use Curated BLAST to search for 1.1.1.140
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZSM8 at UniProt or InterPro
Protein Sequence (252 amino acids)
>Pf6N2E2_925 Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (Pseudomonas fluorescens FW300-N2E2) MNSTGNTILVTGGTSGIGLGLALRLHKAGNKVIIAGRRKALLEKIVSEHPGIESVLLDVS DPQSIQRNSEALAISYPNLNVLINNAGIMHWEDLTDPENLSTAEDIVTTNLLGTIRMVYA FTPQLIKQPSATIMNVSSALAFVPLPATPTYSATKAAVHSFTQSLRVQLEASSVEVVELA PPGVRTTLLGQENDEHAMPLEEFLDEIFELLEISPTPPELVVERAKPLRFAEASGAHDEV LKMLAGYKPPAE