Protein Info for Pf6N2E2_805 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): Xylulose kinase (EC 2.7.1.17)
Rationale: Specifically important for utilizing D-Xylose. Automated validation from mutant phenotype: the predicted function (XYLULOKIN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Xylulose kinase (EC 2.7.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF00370: FGGY_N" amino acids 7 to 255 (249 residues), 236.1 bits, see alignment E=4.7e-74 TIGR01312: xylulokinase" amino acids 8 to 492 (485 residues), 611.8 bits, see alignment E=3.7e-188 PF02782: FGGY_C" amino acids 321 to 446 (126 residues), 80.3 bits, see alignment E=1.7e-26

Best Hits

Swiss-Prot: 51% identical to XYLB_HAEIN: Xylulose kinase (xylB) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K00854, xylulokinase [EC: 2.7.1.17] (inferred from 98% identity to pba:PSEBR_a3091)

MetaCyc: 74% identical to xylulose kinase (Pseudomonas fluorescens)
Xylulokinase. [EC: 2.7.1.17]

Predicted SEED Role

"Xylulose kinase (EC 2.7.1.17)" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization or Xylose utilization (EC 2.7.1.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZTM9 at UniProt or InterPro

Protein Sequence (495 amino acids)

>Pf6N2E2_805 Xylulose kinase (EC 2.7.1.17) (Pseudomonas fluorescens FW300-N2E2)
MANQQLFLGIDCGTQGTKALILDATSGQVLGLGAAAHSMISGANGRREQDTQQWLDAFTQ
ATHQALAAAGVDGQAILGIGVSGQQHGLVLLDDQGQVLRPAKLWCDTETTPENDRLLAHL
GGEDGSLERLGVVIAPGYTVSKLLWTREQHPQVFERIASVLLPHDFLNYWLTGRHCSEYG
DASGTGYFNVRTRQWDVQLLQHIDPSARLQAALPELIEAHQPVGRILPAIAAHLGINPEA
VVASGGGDNMMGAIGTGNIQPGVITMSLGSSGTVYAYAAEPAVSPQPSVATFCSSSGGWL
PLICTMNLTNATGAIRELLDLDIDAFNALVAKAPIGAEGVCMLPFLNGERVPALPHATGS
LLGLTTTNLTRANLCRAVVEGTTFGLRYGLDLLRANGLQAQSIRLIGGGSKSAQWRQIVA
DTMDTTVICTEQSEAAALGAAIQAAWCHSGSQTGLAELCERCVKLDPSSETRPIAAHVAA
SQQAYERYRQHVATL