Protein Info for Pf6N2E2_802 in Pseudomonas fluorescens FW300-N2E2

Annotation: Threonine dehydratase biosynthetic (EC 4.3.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01124: threonine ammonia-lyase, biosynthetic
  • amino acids 14 to 332 (319 residues), 495 bits, E=1.2e-152
• PF00291: PALP
  • amino acids 28 to 314 (287 residues), 272.5 bits, E=2.4e-85

Best Hits

Swiss-Prot: 39% identical to Y4TJ_SINFN: Putative threonine dehydratase (NGR_a01490) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 97% identity to pba:PSEBR_a3094)

Predicted SEED Role

"Threonine dehydratase biosynthetic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.3.1.19)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-methionine degradation II (3/3 steps found)
• superpathway of L-threonine metabolism (13/18 steps found)
• L-threonine degradation I (4/6 steps found)
• L-threonine degradation V (1/2 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.19

Protein Sequence (335 amino acids)

>Pf6N2E2_802 Threonine dehydratase biosynthetic (EC 4.3.1.19) (Pseudomonas fluorescens FW300-N2E2)
MTVTRTEQTLLEHYVKKILAAPVYELAVRTPLQAAPALSEALGNRILLKREDLQPTFSFK
IRGAYNKLVQLTPEQRARGVITASAGNHAQGVALAARELGISASIVMPVTTPQLKVLGVR
NRGAEAILHGESFPFALAHALELAEQSGWEFVSPFDDPDVIAGQGTVAMEILRQHPGQLD
AIFVPVGGGGLIAGIAAYVKYLRPEVRIIGVESQHSACLQAALAAGERVTLPEVGTFADG
VAVAQIGAHGLDICRFCVDEVMTVSNDQLCAAIKDIYDDTRSITEPSGALAVAGIKQYVA
GTGARGQTLVAIDSGANINFDSLRHVAERAAVSAF