Protein Info for Pf6N2E2_797 in Pseudomonas fluorescens FW300-N2E2

Annotation: ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 655 TIGR02779: DNA ligase D, ligase domain" amino acids 35 to 334 (300 residues), 304 bits, see alignment E=1.6e-94 PF01068: DNA_ligase_A_M" amino acids 42 to 215 (174 residues), 67.6 bits, see alignment E=2.4e-22 TIGR02776: DNA ligase D" amino acids 44 to 635 (592 residues), 685.6 bits, see alignment E=1.1e-209 PF04679: DNA_ligase_A_C" amino acids 233 to 324 (92 residues), 72.2 bits, see alignment E=7.7e-24 TIGR02778: DNA ligase D, polymerase domain" amino acids 362 to 605 (244 residues), 291.7 bits, see alignment E=7.6e-91 PF21686: LigD_Prim-Pol" amino acids 379 to 631 (253 residues), 300.3 bits, see alignment E=2.5e-93

Best Hits

KEGG orthology group: None (inferred from 93% identity to pba:PSEBR_a3098)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZTR5 at UniProt or InterPro

Protein Sequence (655 amino acids)

>Pf6N2E2_797 ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD (Pseudomonas fluorescens FW300-N2E2)
MANAESESARLSNAKSEATTARKAPQNRLPERLSPQLATPVEEPPTGEWRYEVQFHGYRL
LARIEQGEVRLFNRNHQDWTERLKLHADALAGWNLGDSWLDGELVLLDETGRSDFAALRQ
AFDISRTVDMLYFLFDAPFLNGVDLRQAPVEERRAALKKALGNNASKRLRFSESFSASPH
DMFESAIALALDSVVGKRVGSPYRSQRSADWVKLKCRLRQAFVIVGFTRPQGRRSGFGAL
LLAVNGPSGLVYAGRVGTGFSQTQIQQLHAQLCAQQRETSPLALPLNGVQGRGVQWVEPG
LTCEVEFIEWTGDNQVRQAIFLGFSTETPANKVVREQPLPIKSTTAKTQRRSKTRAAKVD
GVTITHPDRVIDSVSGVQKAELAQYYLDIAPWILPHLKQRPVALLRAPDGIGEEQFFQKH
ADRLDIPHIKQLDPRLDPGHAALMEIDDIHALVSAAQMGTVELHTWTATHDRIETPDLFV
LDLDPDPSLPWSAMIEATHMTLAVLDELGLQAFLKTSGGKGMHVIVPLARSEGWDTTKAF
AKAISQFLTRQMPERITATMGQKNRVGKIFVDYLRNARGASTVAAYSVRARPGLPVSVPI
ARAELPTLRNAQQWDIYTALERAKGLGADPWEGYHHRQRITARMWEQLDAQKPEA