Protein Info for Pf6N2E2_777 in Pseudomonas fluorescens FW300-N2E2

Annotation: FMN-dependent NADH-azoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 PF02525: Flavodoxin_2" amino acids 1 to 198 (198 residues), 174.4 bits, see alignment E=2.5e-55 PF03358: FMN_red" amino acids 1 to 147 (147 residues), 51.8 bits, see alignment E=7e-18

Best Hits

Swiss-Prot: 78% identical to AZOR3_PSEPF: FMN-dependent NADH-azoreductase 3 (azoR3) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01118, FMN-dependent NADH-azoreductase [EC: 1.7.-.-] (inferred from 98% identity to pba:PSEBR_a3103)

Predicted SEED Role

"FMN-dependent NADH-azoreductase"

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZTQ0 at UniProt or InterPro

Protein Sequence (202 amino acids)

>Pf6N2E2_777 FMN-dependent NADH-azoreductase (Pseudomonas fluorescens FW300-N2E2)
MKLLHIDSSILGDNSASRQLSRQVVEAWKAAEPGITVTYRDLAADAISHFSAQTLVAAGT
SAELRDAALKYEADLSTETMAEFQAADALVIAAPMYNFSIPTQLKAWIDRIAVAGQTFRY
TEAGFEGLCGGKKLVIVSTAGGLHAGQPSGAGHEDYLKVMFGFLGITDIEFVRAEGLAKG
DDMRNKGMNEAQAQIGEQFAAA