Protein Info for Pf6N2E2_744 in Pseudomonas fluorescens FW300-N2E2
Annotation: Trans-feruloyl-CoA synthase (EC 6.2.1.34)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K12508, feruloyl-CoA synthase [EC: 6.2.1.34] (inferred from 98% identity to pba:PSEBR_a3134)MetaCyc: 49% identical to 4-coumarate-CoA ligase (Rhodopseudomonas palustris CGA009)
4-coumarate--CoA ligase. [EC: 6.2.1.12]
Predicted SEED Role
"Trans-feruloyl-CoA synthase (EC 6.2.1.34)" (EC 6.2.1.34)
MetaCyc Pathways
- ferulate degradation (3/3 steps found)
- 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
- umbelliferone biosynthesis (2/3 steps found)
- naringenin biosynthesis (engineered) (2/4 steps found)
- xanthohumol biosynthesis (2/4 steps found)
- 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
- 4-coumarate degradation (aerobic) (2/5 steps found)
- flavonoid biosynthesis (2/5 steps found)
- chlorogenic acid biosynthesis II (1/5 steps found)
- phaselate biosynthesis (1/5 steps found)
- 4-coumarate degradation (anaerobic) (1/6 steps found)
- rosmarinic acid biosynthesis I (3/10 steps found)
- chlorogenic acid biosynthesis I (1/8 steps found)
- avenanthramide biosynthesis (1/9 steps found)
- flavonoid di-C-glucosylation (5/15 steps found)
- superpathway of rosmarinic acid biosynthesis (4/14 steps found)
- curcuminoid biosynthesis (1/10 steps found)
- coumarins biosynthesis (engineered) (3/13 steps found)
- monolignol biosynthesis (1/15 steps found)
- suberin monomers biosynthesis (3/20 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Phenylpropanoid biosynthesis
- Ubiquinone and menaquinone biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.2.1.12 or 6.2.1.34
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165ZUS1 at UniProt or InterPro
Protein Sequence (625 amino acids)
>Pf6N2E2_744 Trans-feruloyl-CoA synthase (EC 6.2.1.34) (Pseudomonas fluorescens FW300-N2E2) VSSESRSSSRPEAGRYRQVSIGHPAVEVREERGILHMRSLEPLAPLPARLLERLVHWAKV RPEQTFIAAREAGGDWRRVSYAQMLDSVRAIAQSLLGYGLSAEKPLALLSGNDIEHLQMA LGAMYAGIPYCPVSPAYSLLSQDFAKLRHVCDLLQPGLVFVSEAAPFERAITAVLPADVP LITVRGEIAGRSRTSFASLLVQPGGAEAEQAFAATGPDSIAKFLFTSGSTKLPKAVITTQ RMLCANQQMLLQTFPVFGEAPPVLVDWLPWNHTFGGSHNVGIVLYNGGTFYLDDGKPTAQ GFAETLRNLKEISPTAYLTVPKGWEELVNALEQDDELRERFFKRISLFFFAAAGLSQSTW DRLDKVAEQHCGERIRMMAGLGMTEAAPSCTFTTGPLSMAGYIGLPAPGCEVRLVPVDGK FEGRFRGPHIMPGYWRSPQQTAEVFDEDGFYCSGDAIKLADASNPQLGLMFDGRIAEDFK LSSGVFVSVGPLRNRAVLEGTPYVQDLVITAPDRECLGALVFPRLAECRRLSGLGGDASD AQVLASPTVRQWFADWLRRLNREASGNASRVEWIALLDEPASIDRGEITDKGSINQRAVL QWRAGKVEALYRGEDASILRAGPVA