Protein Info for Pf6N2E2_735 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 PF03477: ATP-cone" amino acids 15 to 97 (83 residues), 66.4 bits, see alignment E=2.7e-22 TIGR02487: anaerobic ribonucleoside-triphosphate reductase" amino acids 126 to 658 (533 residues), 459.4 bits, see alignment E=9e-142 PF13597: NRDD" amino acids 127 to 658 (532 residues), 582.9 bits, see alignment E=7.6e-179

Best Hits

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 99% identity to pba:PSEBR_a3142)

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZS84 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Pf6N2E2_735 Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2) (Pseudomonas fluorescens FW300-N2E2)
MQSTLISVGCNRLHKRDGSVVAFDADKIRQALIAAGKATGEYAEAEAEALLEAVLARLEG
QTRLNVEQIQDRVERVLMDAGFFLSMRAYIVYREQHGRLRRDRRTLVEVATSMNEYLDRE
DWRVQANANQGYSLGGLILNVSGKVTANYWLDEVYSQAIGQAHREADLHIHDLDMLAGYC
AGWSLRTLLHEGLNGVPGRVEAGPPKHLSSALGQMVNFLGTLQNEWAGAQAFSSFDTYLA
PYVRKDQLSYDEVRQSLQEFIYNLNVPSRWGTQTPFTNLTFDWVCPQDLREQIPIIGGEE
MPFAYGDLQAEMELINRAYIEVMQAGDAKGRVFTFPIPTYNITHDFPWDSENADRLFEMT
ARYGLPYFQNFLNSDMQPNQVRSMCCRLQLDVRELLKRGGGLFGSAEQTGSLGVVTINCA
RLGYLYKGDTSGLLQRIDTLMELAKESLEVKRKVIQHHMDAGLYPYTKRYLGTLRNHFST
IGVNGLHEMLRNFTDDQQGLHTEQGRAFALKLLDHVRATLLRFQEETGHLYNLEATPAEG
TTYRFAKEDLKRYPDILQAGSPVAPYYTNSSQLPVGFTEDPFEALELQDELQCKYTGGTV
LHLYMAEQISSTQACKQLVRKALGRFRLPYLTITPTFSICPVHGYLAGEHEFCPKCDEAL
LLQQQRQGSVH