Protein Info for Pf6N2E2_729 in Pseudomonas fluorescens FW300-N2E2

Annotation: Respiratory nitrate reductase alpha chain (EC 1.7.99.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1257 TIGR01580: nitrate reductase, alpha subunit" amino acids 2 to 1239 (1238 residues), 2567.9 bits, see alignment E=0 PF14710: Nitr_red_alph_N" amino acids 3 to 40 (38 residues), 61.5 bits, see alignment (E = 1.2e-20) PF00384: Molybdopterin" amino acids 108 to 835 (728 residues), 229.2 bits, see alignment E=1.2e-71 PF01568: Molydop_binding" amino acids 1091 to 1206 (116 residues), 72.4 bits, see alignment E=4.7e-24

Best Hits

Swiss-Prot: 70% identical to NARZ_ECOLI: Respiratory nitrate reductase 2 alpha chain (narZ) from Escherichia coli (strain K12)

KEGG orthology group: K00370, nitrate reductase 1, alpha subunit [EC: 1.7.99.4] (inferred from 68% identity to aeh:Mlg_1003)

MetaCyc: 87% identical to respiratory nitrate reductase alpha subunit (Stutzerimonas stutzeri)
RXN-11236 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase alpha chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZU34 at UniProt or InterPro

Protein Sequence (1257 amino acids)

>Pf6N2E2_729 Respiratory nitrate reductase alpha chain (EC 1.7.99.4) (Pseudomonas fluorescens FW300-N2E2)
VSHLLDQLRFFNRKQGEFSDGHGETRKESRDWENVYRSRWQYDKIVRSTHGVNCTGSCSW
KIYVKNGLITWETQQTDYPRTRNDLPNHEPRGCPRGASYSWYIYSANRLKYPKIRKPLLK
LWREARQTLPPVEAWASIVENKAKADSYKSKRGMGGFIRSNWEEVNEIIAAANVYTVKTH
GPDRVVGFSPIPAMSMVSYAAGSRYLSLIGGVCLSFYDWYCDLPPASPMVWGEQTDVPES
ADWYNSNYIIAWGSNVPQTRTPDAHFFTEVRYKGTKTVAITPDYSEVAKLTDLWLNPKQG
TDAALAQAFNHVIFKEFHLDKPSAYFTEYAKRYTDLPVLVMLKPMLGAAPGAGYQPDRFL
RASDLTDNLGQENNPEWKTIAMDAAGELVSPQGSIGYRWGEKGKWNILPREGGEGREIDL
KLSLIGGDVAEVAFPYFAGEAQEYFQHVAGDAVQFRRVPVHSVVLADGSVAKVATVFDLS
AANLAIDRGLGGANVAKDYDDASVPGTPAWQEQITGVSREKAIQIAREFADNADKTRGRS
MIIVGAAMNHWYHMDMNYRGLINMLMLCGCVGQTGGGWAHYVGQEKLRPQCGWLPLAFGL
DWNRPPRQMNGTSFFYGHSSQWRHEKMSMHDVLSPLADKSQFPEHALDYNIRAERAGWLP
SAPQLNTNPLHICRDAAAAGLEPKDYVVKSLQDGSLRFACEQPDSPVNFPRNMFIWRSNL
LGSSGKGHEYMLKYLLGTKNGVMNEDIGHSTECKPTEAEWVDEGAIGKLDLVTTLDFRMS
STCVYSDIVLPTATWYEKDDMNTSDMHPFIHPLSAAIDPAWESRSDWEIYKGIAKAFSAM
SVGHLGVEKDLVTVPLMHDSVGELAQPFGGTDWKSAGVAPVPGKNAPNLQVVERDYPNIY
KQFTSLGPMLEKLGNGGKGINWNTETEVKFLGELNHKEVEAGISQGRPKIDSAIDAAEVI
LSLAPETNGHVAVKAWAALSEFTGIDHSHLAVSKEHEAIRFRDIQAQPRKIISSPTWSGL
EDDHVSYNAGYTNVHESIPWRTITGRQQFYQDHPWMQAFGEQLMSYRPPVNTRTIAGVKG
KRSNGETEIVLNWITPHQKWGIHSTYSDNLLMLTLSRGGPIVWLSEIDAKRAGIEDNDWI
ECFNVNGALTARAVVSQRVKEGMVMMYHAQERIVNVPGSETTKTRGGHHNSVTRVVLKPT
HMIGGYAQQAYGFNYYGTVGCNRDEFVVVRKMVKVDWLDGSSGDDLPRPLPTDIEEN