Protein Info for Pf6N2E2_727 in Pseudomonas fluorescens FW300-N2E2

Annotation: Nitrate/nitrite transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 9 to 33 (25 residues), see Phobius details amino acids 46 to 64 (19 residues), see Phobius details amino acids 74 to 92 (19 residues), see Phobius details amino acids 97 to 122 (26 residues), see Phobius details amino acids 132 to 156 (25 residues), see Phobius details amino acids 162 to 181 (20 residues), see Phobius details amino acids 211 to 230 (20 residues), see Phobius details amino acids 241 to 262 (22 residues), see Phobius details amino acids 274 to 291 (18 residues), see Phobius details amino acids 311 to 332 (22 residues), see Phobius details amino acids 348 to 371 (24 residues), see Phobius details amino acids 377 to 398 (22 residues), see Phobius details PF07690: MFS_1" amino acids 16 to 293 (278 residues), 127.9 bits, see alignment E=2.2e-41

Best Hits

Swiss-Prot: 41% identical to NARK1_THETH: Probable nitrate/nitrite antiporter NarK1 (narK1) from Thermus thermophilus

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 99% identity to pba:PSEBR_a3150)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YW71 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Pf6N2E2_727 Nitrate/nitrite transporter (Pseudomonas fluorescens FW300-N2E2)
MRAQVQQGLVLGMSTLAFTVCFMVWMMFAVLGVPIKELLALNETQFGLLAATPVLTGSLV
RLPLGLLTDRFGGRIVFFLLMLSCVLPLYLITLATAFWQFLVLGLFVGLAGGSFSVGIAY
VAKWFDKQNQGFAMGVFGAGNAGAAVTKFLAPALIAFGSWHLVPKVFSAILFITALLFWF
LTSEKKEHSGTGGATLREQLKTLKEPAVWRYCQYYSIVFGGYVALALWMTKYYVQEYGFS
LQSAALLAACFSLPGGVLRAVGGWMSDRWGAQSVTWWVLWVSWICLFLLSYPQTQLQVQT
VNGIVDFHIGLSPTLFTVLLFVMGIAFAFGKASVFKYIANDYPKNMGAVSGIVGLAGGLG
GFVLPIMFGALVDLTGVRSSCFMLMYGVVWVSLAWMYFSEMRKRPLLGKQPPATQSPFSS
IAQGEEHVRSANA