Protein Info for Pf6N2E2_705 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cytochrome cd1 nitrite reductase (EC:1.7.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF13442: Cytochrome_CBB3" amino acids 51 to 125 (75 residues), 40.8 bits, see alignment E=3.5e-14 PF00034: Cytochrom_C" amino acids 55 to 127 (73 residues), 24.7 bits, see alignment E=7.3e-09 PF02239: Cytochrom_D1" amino acids 162 to 554 (393 residues), 512.7 bits, see alignment E=6.3e-158

Best Hits

Swiss-Prot: 78% identical to NIRS_PSEAE: Nitrite reductase (nirS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a3172)

MetaCyc: 65% identical to nitrite reductase (NO-forming) monomer (Cupriavidus necator)
Nitrite reductase (NO-forming). [EC: 1.7.2.1]

Predicted SEED Role

"Cytochrome cd1 nitrite reductase (EC:1.7.2.1)" in subsystem Denitrification or Dissimilatory nitrite reductase or Heme biosynthesis orphans (EC 1.7.2.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZS60 at UniProt or InterPro

Protein Sequence (559 amino acids)

>Pf6N2E2_705 Cytochrome cd1 nitrite reductase (EC:1.7.2.1) (Pseudomonas fluorescens FW300-N2E2)
MLISHKKSWALRVTALCTALAAPHAFADQTAAPEQPRVVKTEGAPDLSQADFDTAKEIYF
QRCAGCHGVLRKGATGKPLTPDITQSRGQAYLEALITYGSAAGMPNWGTSNALTKDQITV
MAKFIQHTPPTPPEWGMAETCKTWKVLVKPEDRPTKQISTLNLQNLFSVTLRDDGKIALI
DGDSKKIVKLIDTGYAVHISRISASGRYLLVIGRDAKIDMIDLWPKEPTKVAEIKVGIEA
RSVETSKFKGYEDKYTIAGSYWPPQFTIMDGETLEPKQIVSTRGMTVDTQQYHPEPRVAA
IIASHEWPEFIVNVKETGKVMLVNYQDIKNLTITSIDAAPFLHDGGWDSSHRYFMTAANN
SNKVAVIDSKERKLTALVDVGKTPHPGRGANFNHPLYGPVWATSHLGDAGVSLIGTDPVN
HPQYAWKQVGSLQGQGGGSLFIKTHPASRHLYVDTTLSPDAKLSQSVAVFNIDKLDAGYT
VLPIAEYAGIKKGALRVVQPEYNKAGDEVWFSVWSGQEDESALVVIDDKTLKVKNVIKDK
RLITPTGKFNVYNTQHDIY