Protein Info for Pf6N2E2_680 in Pseudomonas fluorescens FW300-N2E2
Annotation: D-amino-acid oxidase (EC 1.4.3.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a3197)MetaCyc: 68% identical to D-hydroxyproline dehydrogenase beta subunit (Pseudomonas aeruginosa PAO1)
1.14.19.-
Predicted SEED Role
"D-amino-acid oxidase (EC 1.4.3.3)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 1.4.3.3)
MetaCyc Pathways
- trans-4-hydroxy-L-proline degradation II (4/4 steps found)
- superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
- L-lysine degradation V (6/9 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (4/7 steps found)
- butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
- superpathway of L-lysine degradation (19/43 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- D-Arginine and D-ornithine metabolism
- Glycine, serine and threonine metabolism
- Penicillin and cephalosporin biosynthesis
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.3.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165YVU7 at UniProt or InterPro
Protein Sequence (371 amino acids)
>Pf6N2E2_680 D-amino-acid oxidase (EC 1.4.3.3) (Pseudomonas fluorescens FW300-N2E2) VSEGHVADVIIIGAGIIGAACAQALARRNVNVLVLDAGWHGATAAGMGHLLVLDDNPAEL ALSQYSLQRWRELAPALPDGCAWRNNGTLWLAANAEEMAVAHSKYLNLLAHGEACELIGH AALHQREPELRPGLEGGLLIKGDGILYAPAAARWMLDAPNIRQQRAQVSEVDGHRVRLDD GRWLSAEAVVLANGIQATELCPELPIVPKKGHLLITDRYPATVTHTLVELGYVTSAHNAS GPSVACNIQPRPTGQLFIGASRQFGTLDPQVEGWMLAKMLKRAVEYLPGLAHLNGIRAWT GFRAASPDGLPLVGQHPQRQGLWLAVGHEGLGVTTAPATADLLVAQLFNETSPLAPQAYL PQRFLGEPAHA