Protein Info for Pf6N2E2_656 in Pseudomonas fluorescens FW300-N2E2

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 857 transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 264 to 287 (24 residues), see Phobius details PF05228: CHASE4" amino acids 74 to 228 (155 residues), 57.6 bits, see alignment E=5e-19 TIGR00229: PAS domain S-box protein" amino acids 310 to 428 (119 residues), 40.5 bits, see alignment E=2.8e-14 PF00989: PAS" amino acids 314 to 364 (51 residues), 23.2 bits, see alignment 1.8e-08 PF08448: PAS_4" amino acids 320 to 425 (106 residues), 25.4 bits, see alignment E=4.4e-09 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 433 to 594 (162 residues), 151 bits, see alignment E=2.6e-48 PF00990: GGDEF" amino acids 437 to 590 (154 residues), 165.7 bits, see alignment E=2.2e-52 PF00563: EAL" amino acids 611 to 839 (229 residues), 180.2 bits, see alignment E=1.4e-56

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a3223)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZTB6 at UniProt or InterPro

Protein Sequence (857 amino acids)

>Pf6N2E2_656 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens FW300-N2E2)
MIEIAGNDSTVLSPGSPTRSFTRRTFPAIMLLLFVMSALAIAVLLNITGVQDRRAREQSL
FFAQRAVEGIRKGIGRDLSDYSKWSDAYRHLHVRVDKEWAYDQENVGSSVYSLYGYQAVF
VISPTGQTVYSVIDGQMSEVNADTWLTGDLRALLKKAAAAQNHDEVVVEMLHHEDAPAYV
AASAITIGTDSSVPEIPGPPSLMLFVKVLDPGALENLARDFALPDAHIARTPGPADTAQL
LLDNLTLEALAWRPQTPGQDLRKILLPLLAVALFVLGILALAVLRHALAMLRAQERQYAS
LLAHRKALERSEERFRDIAEVSSDWLWEVDSTGTLTYLSERFEQVTGFSPTQWLGKPLHR
LLHPHGGSISIAQWLLGGANSDSSAPLLCEYTARNQRIRTCKLSVRAIEAGTLGFRGTAT
DITDELRALAQIKHLSLHDPLTGLANRNRLFDCLREHLDRADRRPLALLNLDMDRFKPVN
DSLGHAVGDKVLKEVAHILQQNVRGTDLVARLGGDEFVIVMPDPGDADDLDQLCERLIDC
MQRPMHLDGNTLYLGVSIGVAWSQPGDERADELLRHADIALYAAKAAGRNTWRVYVEAMG
NLARDRRQYEQQLRDAMQRDQLELRYLPRFDVSAEQLHGFEAQTFWHHPEKGELGGADFI
PVAETSGQLEELGTWMLINVCEEAATWPSAVNVSIAVSPRWFNSSFLLDQVQEALQASGL
APHRLTLEVAEGVLLADHKTVASTLHALKDLGVRINIDKFGTSIASLREVLDQPFDGIRF
DRNILTQLGLEDDKEGVLAMIRLSRSVGLMVTAEGIENARQFSQLRNVACDHVQGPYFGA
ALARSEMASFFTTPRWL