Protein Info for Pf6N2E2_634 in Pseudomonas fluorescens FW300-N2E2
Annotation: NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to DHE2_PSEAE: NAD-specific glutamate dehydrogenase (gdhB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Predicted SEED Role
"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)
MetaCyc Pathways
- L-alanine degradation II (to D-lactate) (3/3 steps found)
- L-glutamate degradation I (1/1 steps found)
- ethene biosynthesis IV (engineered) (1/3 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
- 4-aminobutanoate degradation V (2/7 steps found)
- methylaspartate cycle (9/19 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZTW6 at UniProt or InterPro
Protein Sequence (1622 amino acids)
>Pf6N2E2_634 NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form (Pseudomonas fluorescens FW300-N2E2) MAFFTAASKADFQHQLQAALAQHISEQALPQVALFAEQFFGIISLDELTQRRLSDLAGCT LSAWRLLERFDHAQPQVRVYNPDYERHGWQSTHTAVEVLHHDLPFLVDSVRTELNRRGYS IHTLQTTVLSVRRGSKGELLEILPKGTQGDDILQESLMYLEIDRCANAAELNVLSKELEQ VLGEVRVAVADFEPMKAKVQEILDSLDSSAYAIEADEKSEIKSFLEWLVGNHFTFLGYEE FVVRDEADGGHIEYNPDSFLGLTKLLRAGLTAEDLRIEDYAVNYLREPTPLSFAKAAHPS RVHRPAYPDYVSIREIDADGKVIKECRFMGLYTSSVYGESVRAIPYIRRKVAEIERRSGF QAKAHLGKELAQVVEVLPRDDLFQTPVDELFSTVMSIVQIQERNKIRVFLRKDPYGRFCY CLAYVPRDIYSTEVRQKIQQVLMDRLKASDCEFWTFFSESVLARVQLILRVDPKNRIDID PLLLEKEVVQACRSWKDDYASLVVESFGEAQGTNVLSDFPKGFPAGYRERFAAHSAVVDM QHLLSLSEKNPLVMSFYQPLGQVSGQRELHCKLYHADTPLALSDVLPILENLGLRVLGEF PYRLRHTNGREFWIHDFAFTAAEGLDLDIQQLNDTLQDAFVHIVRGDAENDAFNRLVLTA GLPWRDVALLRAYARYLKQIRLGFDLGYIASTLNNHTDIARELTRLFKTRFYLARKLTGD DLEDKQLRLEQAILSALDDVQVLNEDRILRRYLDLIKATLRTNFYQTDANGQNKSYFSFK FNPHLIPELPKPVPKFEIFVYSPRVEGVHLRFGNVARGGLRWSDREEDYRTEVLGLVKAQ QVKNSVIVPVGAKGGFLPRRLPLGGSRDEIAAEGIACYRIFISGLLDITDNLKDGALVPP ANVVRHDDDDPYLVVAADKGTATFSDIANGIAIDYGFWLGDAFASGGSAGYDHKKMGITA KGAWVGVQRHFRERGINVQEDSITVVGVGDMAGDVFGNGLLMSDKLQLVAAFNHLHIFID PNPEPASSFAERQRLFDLPRSAWTDYDTSIMSEGGGIFSRSAKSIAISPQMKERFDIKAD KLTPTELLNALLKAPVDLLWNGGIGTYVKASTESHADVGDKANDALRVNGNELRCKVVGE GGNLGMTQLGRVEFGLNGGGSNTDFIDNAGGVDCSDHEVNIKILLNEVVQAGDMTDKQRN QLLASMTDEVGGLVLGNNYKQTQALSLAARRAFVRIAEYKRLMNDLEARGKLDRAIEFLP TEEQLAERVAAGHGLTRAELSVLISYSKIDLKEALLNSLVPDDDYLTRDMETAFPPTLVS KFSEAMRRHRLKREIVSTQIANDLVNHMGITFVQRLKESTGMSPANVAGAYVIVRDIFHL PHWFRQIEALDHQVSADVQLELMDELMRLGRRATRWFLRSRRNEQNAARDVAHFGPHLAA LGLKLDELLEGPTREGWQTRYQAYVAAGVPELLARMVAGTTHLYTLLPIIEASDVTGQNA ADVAKAYFAVGSALDITWYLQQISALPVENNWQALAREAFRDDVDWQQRAITISVLQEGD GAQDVETRLALWLEQHHEMVERWRAMLVDIRAASGTDYAMYAVANRELLDVAMSGQAVVT AN