Protein Info for Pf6N2E2_5970 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): ABC transporter for D-galactose/L-arabinose, permease component
Rationale: Specifically important for utilizing D-galactose and L-arabinose
Original annotation: L-arabinose transport system permease protein (TC 3.A.1.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 transmembrane" amino acids 24 to 44 (21 residues), see Phobius details amino acids 52 to 72 (21 residues), see Phobius details amino acids 78 to 95 (18 residues), see Phobius details amino acids 101 to 124 (24 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details amino acids 169 to 190 (22 residues), see Phobius details amino acids 221 to 241 (21 residues), see Phobius details amino acids 255 to 288 (34 residues), see Phobius details amino acids 300 to 318 (19 residues), see Phobius details PF02653: BPD_transp_2" amino acids 56 to 314 (259 residues), 123.1 bits, see alignment E=6e-40

Best Hits

Swiss-Prot: 62% identical to ARAH_ECOLI: L-arabinose transport system permease protein AraH (araH) from Escherichia coli (strain K12)

KEGG orthology group: K10538, L-arabinose transport system permease protein (inferred from 99% identity to pba:PSEBR_a4033)

MetaCyc: 62% identical to arabinose ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose transport system permease protein (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GM94 at UniProt or InterPro

Protein Sequence (322 amino acids)

>Pf6N2E2_5970 ABC transporter for D-galactose/L-arabinose, permease component (Pseudomonas fluorescens FW300-N2E2)
MTIQNNALPTARKPLDLRRFLDDWVMLLAAIGIFVLCTLMIDNFLSPLNMRGLGLAISTT
GIAACTMLYCLASGHFDLSVGSVIACAGVVAAVVMRDTNSVFLGISAALVMGLIVGLING
IVIAKLRVNALITTLATMQIVRGLAYIFANGKAVGVSQESFFVFGNGQMFGVPVPILITI
VCFLFFGWLLNYTTYGRNTMAIGGNQEAALLAGVNVDRTKIIIFAVHGVIGALAGVILAS
RMTSGQPMIGQGFELTVISACVLGGVSLSGGIGMIRHVIAGVLILAIIENAMNLKNIDTF
YQYVIRGSILLLAVVIDRLKQR