Protein Info for Pf6N2E2_596 in Pseudomonas fluorescens FW300-N2E2

Annotation: Arsenic efflux pump protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 32 to 53 (22 residues), see Phobius details amino acids 60 to 82 (23 residues), see Phobius details amino acids 94 to 156 (63 residues), see Phobius details amino acids 175 to 198 (24 residues), see Phobius details amino acids 222 to 242 (21 residues), see Phobius details amino acids 248 to 266 (19 residues), see Phobius details amino acids 278 to 296 (19 residues), see Phobius details amino acids 316 to 334 (19 residues), see Phobius details amino acids 401 to 423 (23 residues), see Phobius details TIGR00935: arsenite/antimonite efflux pump membrane protein" amino acids 3 to 426 (424 residues), 680.4 bits, see alignment E=6e-209 PF02040: ArsB" amino acids 4 to 423 (420 residues), 487.7 bits, see alignment E=4.2e-150 PF03600: CitMHS" amino acids 13 to 368 (356 residues), 214.1 bits, see alignment E=3e-67

Best Hits

Swiss-Prot: 73% identical to ARSB2_ECOLX: Arsenical pump membrane protein (arsB) from Escherichia coli

KEGG orthology group: K03893, arsenical pump membrane protein (inferred from 99% identity to pba:PSEBR_a3279)

MetaCyc: 73% identical to arsenical pump membrane protein (Shewanella sp. ANA-3)
RXN-22366 [EC: 7.3.2.7]

Predicted SEED Role

"Arsenic efflux pump protein" in subsystem Arsenic resistance

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZRY4 at UniProt or InterPro

Protein Sequence (427 amino acids)

>Pf6N2E2_596 Arsenic efflux pump protein (Pseudomonas fluorescens FW300-N2E2)
MLIASLIFLLTITLVIWQPKGLGVGWSATLGALLALLSGVVQVGDIALVWQIIWNATGTF
VALIIISLLLDEAGFFAWAALHVARWGRGSGRKLFAFMVLLGALVSALFANDGAALILTP
IVISMLLALRFSPAATLAFVMGAGFIADSASLPLVVSNLVNIVSADFFGIGFNRYAAVMV
PVNLVSVAASLAILLWFFRRDIPGDYDPDQLDDPATAIHDKATFYAGWVVLVILLLGCFA
LEPLGIPISAISAVCAALLLAVAARGHKISTRKAMKEAPWQIVIFSLGMYLVVYGLRNAG
LTGYLAHWLDGFAEYGVWGAAMGTGVLTALLSSAMNNLPTVLVGALSIDASQATGVVKEA
MIYANVIGSDLGPKITPIGSLATLLWLHVLERKNIRIGWGYYFKVGIVLTVPVLLVTLAA
LAVRLSF