Protein Info for Pf6N2E2_5957 in Pseudomonas fluorescens FW300-N2E2

Annotation: Thymidylate kinase (EC 2.7.4.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00041: dTMP kinase
  • amino acids 3 to 196 (194 residues), 174.3 bits, E=1.2e-55
• PF02223: Thymidylate_kin
  • amino acids 8 to 196 (189 residues), 168.2 bits, E=1.7e-53

Best Hits

Swiss-Prot: 89% identical to KTHY_PSEPF: Thymidylate kinase (tmk) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00943, dTMP kinase [EC: 2.7.4.9] (inferred from 97% identity to pba:PSEBR_a4046)

MetaCyc: 47% identical to Tmk (Escherichia coli K-12 substr. MG1655)
T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.12, 2.7.4.13, 2.7.4.9]

Predicted SEED Role

"Thymidylate kinase (EC 2.7.4.9)" (EC 2.7.4.9)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.9

Sequence Analysis Tools

See A0A165ZSS5 at UniProt or InterPro

Protein Sequence (210 amino acids)

>Pf6N2E2_5957 Thymidylate kinase (EC 2.7.4.9) (Pseudomonas fluorescens FW300-N2E2)
VTGLFITLEGPEGAGKSTNREYLAERLRAAGIEVVLTREPGGTPLAERIREVLLAPIEEV
MNPDTELLLVFAARAQHLAEVIRPALARGAVVLCDRFTDSTYAYQGGGRGLSVERIGALE
TFVQGDLRPDLTLVFDLPVEIGLARASARGRLDRFELEGQAFFNAVRSAFLNRAKADPAR
YLLIDAAQPLAQVQQSLDGLLPRLLERVRG