Protein Info for Pf6N2E2_5903 in Pseudomonas fluorescens FW300-N2E2
Annotation: 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 44% identical to RIB7_AQUAE: 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase (ribD2) from Aquifex aeolicus (strain VF5)
KEGG orthology group: K00082, 5-amino-6-(5-phosphoribosylamino)uracil reductase [EC: 1.1.1.193] (inferred from 79% identity to pst:PSPTO_0835)MetaCyc: 40% identical to pyrimidine nucleotide reductase (Methanocaldococcus jannaschii)
RXN-10057 [EC: 1.1.1.302]
Predicted SEED Role
"5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)" in subsystem Riboflavin, FMN and FAD metabolism (EC 1.1.1.193)
MetaCyc Pathways
- flavin biosynthesis I (bacteria and plants) (8/9 steps found)
- flavin biosynthesis III (fungi) (8/9 steps found)
- flavin biosynthesis II (archaea) (6/10 steps found)
- toxoflavin biosynthesis (3/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.193
Use Curated BLAST to search for 1.1.1.193 or 1.1.1.302
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165ZS37 at UniProt or InterPro
Protein Sequence (224 amino acids)
>Pf6N2E2_5903 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193) (Pseudomonas fluorescens FW300-N2E2) MKVTVFSQISIDGKLTLGNEASSKPLFQHFDDEDMRFIHKFRGEVDAIMVGRNTIITDDP QLTNRYESGRNPVRIIPTTSLNFPASAKIFSSPEHTIIATTEEARDHKMVEHIRAHGKEV LFAGTARVDFKQLFPMLEARGINHIMVEGGGHLNWQVFNLDLVDEIMLMQLPIIIGGSDT TTLIDGEGFQDIEMTKAFKLHEFEARSHYNFLHFKRDPQQRRAH