Protein Info for Pf6N2E2_5897 in Pseudomonas fluorescens FW300-N2E2

Annotation: COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 TIGR04337: AmmeMemoRadiSam system radical SAM enzyme" amino acids 3 to 286 (284 residues), 251.6 bits, see alignment E=5.1e-79 PF04055: Radical_SAM" amino acids 35 to 183 (149 residues), 69.4 bits, see alignment E=2.2e-23

Best Hits

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a4099)

Predicted SEED Role

"COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GU49 at UniProt or InterPro

Protein Sequence (436 amino acids)

>Pf6N2E2_5897 COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like (Pseudomonas fluorescens FW300-N2E2)
LNYGKGVHSTEETIETEAVFHFAPGERILSLGNIGCMLNCGYCHNWKTSQAKYVTDKDVY
YYTPEQVVETALKHGIRVISWTYNDPVVWHEFILDTARLAKEAGLINLYKSAFFISEEAI
DELLPVIDIFSISLKSISPEYYRKVTTGWVEPVLAGIKKVYDAGKYVEVSTLMVTDISDD
EETARKISQWVLDELGPNVPLHFVRFHPDYKMSNSIRTPVDRLLKARDVARSMGVEHVYL
GNVNDVEGTNTSCNNCNALLVTRYGLNAEIIGLDSKGCCSQCGHDAHFKLLGEHQANVPV
ELREEALSAYEKRKFEWHGDIVSLHAQVLNTEDFEQTVYLRRNYTDGHNSDWKSLTLRPH
ESYRFIIAKARIDETGPEVWLPNGVNSNLHEVFDRAHFPTESIEEIGISQNDITPTIGYE
GKQNMYEQVIKLVSQA