Protein Info for Pf6N2E2_5865 in Pseudomonas fluorescens FW300-N2E2

Annotation: Chaperone protein HtpG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 PF02518: HATPase_c" amino acids 34 to 186 (153 residues), 28.6 bits, see alignment E=2.5e-10 PF13589: HATPase_c_3" amino acids 34 to 159 (126 residues), 37.7 bits, see alignment E=3e-13 PF00183: HSP90" amino acids 226 to 631 (406 residues), 434.4 bits, see alignment E=1.2e-133

Best Hits

Swiss-Prot: 96% identical to HTPG_PSEF5: Chaperone protein HtpG (htpG) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 100% identity to pba:PSEBR_a4129)

MetaCyc: 60% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WQN8 at UniProt or InterPro

Protein Sequence (636 amino acids)

>Pf6N2E2_5865 Chaperone protein HtpG (Pseudomonas fluorescens FW300-N2E2)
MTMSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAVDKLRFEALSKPE
LLEGGAELKIRVSFDKDAKTVTLEDNGIGMSREEVITHLGTIAKSGTADFMKNLSGDQKK
DSHLIGQFGVGFYSAFIVADQVEVFSRRAGLAASEGVHWSSKGEGEFEVATVDKADRGTR
IVLHLKSGEDEFADGWRLRNIIKKYSDHIALPIELPKEVTAAEGEEKPEVEWETVNRASA
LWTRPRTEVKDEEYQEFYKHIAHDYENPLSWSHNKVEGKLEYNSLLYVPARAPFDLYQRE
APRGLKLYVQRVFVMDQAESFLPLYLRFIKGVVDSNDLSLNVSREILQKDPIIDSMKSAL
TKRVLDMLEKLAKNEPEQYKSFWKNFGQVMKEGPAEDFANKEKIAGLLRFASTQGDDGEQ
IVGLADYLARAKEGQDKIYYLTGETYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYL
NEFDGKSFVDVARGDLDLGNLDSEEDKKAAEEVAKSKEGLVERIKTALGDAVSEVRVSHR
LTDSPAILAIGEQDLGLQMRQILEASGQKVPDSKPIFEFNPAHPLVEKLDNEQSEERFGD
LSHILFDQAALAAGDSLKDPAAYVRRLNKLLVELSA