Protein Info for Pf6N2E2_5857 in Pseudomonas fluorescens FW300-N2E2
Annotation: Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 90% identical to DLDH2_PSEPU: Dihydrolipoyl dehydrogenase (lpdG) from Pseudomonas putida
KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 95% identity to pfl:PFL_1720)MetaCyc: 47% identical to glycine cleavage system L protein 1 (Arabidopsis thaliana col)
GCVMULTI-RXN [EC: 1.4.1.27]
Predicted SEED Role
"Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)" in subsystem TCA Cycle (EC 1.8.1.4)
MetaCyc Pathways
- folate transformations III (E. coli) (9/9 steps found)
- folate transformations II (plants) (10/11 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
- glycine biosynthesis II (3/3 steps found)
- glycine cleavage (3/3 steps found)
- glycine degradation (3/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
- folate transformations I (9/13 steps found)
- photorespiration II (6/10 steps found)
- photorespiration I (5/9 steps found)
- photorespiration III (5/9 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (5/9 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycine, serine and threonine metabolism
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.4
Use Curated BLAST to search for 1.4.1.27 or 1.8.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165ZRF4 at UniProt or InterPro
Protein Sequence (481 amino acids)
>Pf6N2E2_5857 Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4) (Pseudomonas fluorescens FW300-N2E2) MTQKFDVVVIGAGPGGYVAAIKAAQLGLTTACIEKYTDKEGKLALGGTCLNVGCIPSKAL LDSSWKFHEAQDGLAIHGIGGVNRENVTIDVPAMVGRKANIVKGLTSGVATLFKANGVTS IQGHGKLLMGKKVEVTKPDGSVEVIEAENVILAPGSRPIDIPPAPVDQNVIVDSTGALEF QSVPKRLGVIGAGVIGLELGSVWSRLGAQVTVLEALDTFLMAADAAVSKEALKTLTKQGL DIKLGARVTGSKVNGEEVVVNYTDANGEQTITFDKLIVAVGRRPVTTDLLAADSGVELDE RGFIKVDDFCATTVPGVFAIGDVVRGMMLAHKASEEGIMVVERIKGHKAQINYDLIPSVI YTHPEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGV HVIGPSAAELVQQGAIGMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHVANRKK R