Protein Info for Pf6N2E2_5839 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glyoxylate carboligase (EC 4.1.1.47)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 TIGR01504: glyoxylate carboligase" amino acids 1 to 587 (587 residues), 1160.5 bits, see alignment E=0 PF02776: TPP_enzyme_N" amino acids 1 to 118 (118 residues), 110.5 bits, see alignment E=6.3e-36 PF00205: TPP_enzyme_M" amino acids 190 to 324 (135 residues), 131.3 bits, see alignment E=3.1e-42 PF02775: TPP_enzyme_C" amino acids 390 to 550 (161 residues), 118.1 bits, see alignment E=4.5e-38

Best Hits

Swiss-Prot: 75% identical to GCL_ECO57: Glyoxylate carboligase (gcl) from Escherichia coli O157:H7

KEGG orthology group: K01608, tartronate-semialdehyde synthase [EC: 4.1.1.47] (inferred from 99% identity to pba:PSEBR_a4156)

MetaCyc: 75% identical to glyoxylate carboligase (Escherichia coli K-12 substr. MG1655)
Tartronate-semialdehyde synthase. [EC: 4.1.1.47]

Predicted SEED Role

"Glyoxylate carboligase (EC 4.1.1.47)" in subsystem Allantoin Utilization or Photorespiration (oxidative C2 cycle) (EC 4.1.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GTD0 at UniProt or InterPro

Protein Sequence (588 amino acids)

>Pf6N2E2_5839 Glyoxylate carboligase (EC 4.1.1.47) (Pseudomonas fluorescens FW300-N2E2)
MRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEGYTR
TKAGNIGVCIGTSGPAGTDMVTGLYSASADSIPILCITGQAPRARLHKEDFQAVDITSIV
KPVTKWATTVLEPGQVPYAFQKAFFEMRSGRPGPVLIDLPFDVQMAEIEFDIDAYQPLPL
AKPAANRVQIEKALAMLNQAERPLLVAGGGIINADASELLVAFAELTGIPVIPTLMGWGT
IPDDHPLMVGMVGLQTSHRYGNATLLKSDVVLGIGNRWANRHTGSVDVYTEGRTFIHVDI
EPTQIGRVFTPDLGIVSDAASALTVFIEVAREWQAAGKLKNRDAWLQDCQQRKASLQRKT
HFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAGAQFLHVYKPRHWINCGQAGPLGW
TIPAALGVVKADPSRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIRQAQ
RGFDMDYCVQLSFDNLNAPELNGYGVDHIAVAEGLGCKALRVFEPSQIAPALRQAEKMIE
EFKVPVIVEIILERVTNISMGTEINAVNEFEDLALVGNDAPTAISLLD