Protein Info for Pf6N2E2_5767 in Pseudomonas fluorescens FW300-N2E2

Annotation: Sodium-dependent transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 39 to 57 (19 residues), see Phobius details amino acids 69 to 92 (24 residues), see Phobius details amino acids 99 to 120 (22 residues), see Phobius details amino acids 126 to 151 (26 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 192 to 215 (24 residues), see Phobius details amino acids 222 to 244 (23 residues), see Phobius details amino acids 257 to 276 (20 residues), see Phobius details amino acids 281 to 303 (23 residues), see Phobius details PF13593: SBF_like" amino acids 36 to 304 (269 residues), 81.8 bits, see alignment E=5.7e-27 PF01758: SBF" amino acids 42 to 219 (178 residues), 154.2 bits, see alignment E=3.5e-49

Best Hits

Swiss-Prot: 55% identical to YOCS_BACSU: Uncharacterized sodium-dependent transporter YocS (yocS) from Bacillus subtilis (strain 168)

KEGG orthology group: K03453, bile acid:Na+ symporter, BASS family (inferred from 96% identity to pfs:PFLU1876)

Predicted SEED Role

"Sodium-dependent transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A436 at UniProt or InterPro

Protein Sequence (321 amino acids)

>Pf6N2E2_5767 Sodium-dependent transporter (Pseudomonas fluorescens FW300-N2E2)
MRALAALSRFVGNTFAYWVLFFAVVAFLQPVWFIGLKSAIVPLLGLVMFGMGLTLKLEDF
AEVARHPWRVALGVVAHFVIMPGVAWLLCQVFHLPPEIAVGVILVGCCPSGTSSNVMTWL
ARGDLALSVAIAAVTTLLAPLLTPALIWLLASAWLPVSFMELFWSILQVVLLPIVLGVVA
QRLLGDKVRHAVEVLPLVSVVSIVIIVTAVVAASQAKIAESGLLIMAVVMLHNSFGFLLG
YFTGHLFKLPLAQRKSLALEVGMQNSGLGAALASAHFSPLAAVPSALFSVWHNISGALLS
TYFRRMSEKQDRELQARPAAD