Protein Info for Pf6N2E2_5742 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cobyric acid synthase (EC 6.3.5.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 PF13500: AAA_26" amino acids 3 to 230 (228 residues), 59.2 bits, see alignment E=7.5e-20 PF01656: CbiA" amino acids 4 to 233 (230 residues), 76.7 bits, see alignment E=2.3e-25 TIGR00313: cobyric acid synthase CobQ" amino acids 4 to 475 (472 residues), 468.9 bits, see alignment E=9.7e-145 PF07685: GATase_3" amino acids 250 to 434 (185 residues), 197.5 bits, see alignment E=2.8e-62

Best Hits

Swiss-Prot: 93% identical to COBQ_PSEPF: Cobyric acid synthase (cobQ) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K02232, adenosylcobyric acid synthase [EC: 6.3.5.10] (inferred from 95% identity to pba:PSEBR_a4240)

MetaCyc: 49% identical to CobQ (Pseudomonas denitrificans (nom. rej.))
Adenosylcobyric acid synthase (glutamine-hydrolyzing). [EC: 6.3.5.10]

Predicted SEED Role

"Cobyric acid synthase (EC 6.3.5.10)" (EC 6.3.5.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A6B4 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Pf6N2E2_5742 Cobyric acid synthase (EC 6.3.5.10) (Pseudomonas fluorescens FW300-N2E2)
MTTVMVQGTTSDAGKSTLVTALCRWVRRQGVSVVPFKPQNMALNSAVTADGGEIGRAQAV
QAQAAGLEPHTDMNPVLLKPNSDTGAQVIIHGRAVTTMNAVAYHDYKAIAMQAVLASHTR
LSAAYPVVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVLLIADINRGGVFAHLVGTLEL
LSPSEQARVKGFVINRFRGDIALLQPGLDWLEARTGKPVVGVLPYVMDLHLEAEDGLDQR
QADKVEQLLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQPIPPADLIILPGSKSVR
SDLAYLRANGWESALQRHLRYGGKLLGICGGLQMLGQQVHDPLGLEGAAGSSAGLGLLAF
ETTLEHEKQLRNVRGRLALEEAEVSGYEIHAGVTTGPALEHAAVYLEDGRCDGAQSLDGQ
IFGTYLHGLFESPQASSALLRWAGLPDVQTVDYHGLRERDIERLADLVARHLDTGLLREL
CGI