Protein Info for Pf6N2E2_5740 in Pseudomonas fluorescens FW300-N2E2

Annotation: Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00380: cobalamin biosynthesis protein CobD
  • amino acids 12 to 273 (262 residues), 190.2 bits, E=2.5e-60
• PF03186: CobD_Cbib
  • amino acids 13 to 287 (275 residues), 319 bits, E=2.6e-99
• PF17113: AmpE
  • amino acids 152 to 228 (77 residues), 26.7 bits, E=3.6e-10

Best Hits

Swiss-Prot: 75% identical to COBD_PSEAE: Cobalamin biosynthesis protein CobD (cobD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02227, adenosylcobinamide-phosphate synthase CobD [EC: 6.3.1.10] (inferred from 96% identity to pba:PSEBR_a4242)

MetaCyc: 35% identical to adenosylcobinamide-phosphate synthase (Salmonella enterica enterica serovar Typhimurium)
Adenosylcobinamide-phosphate synthase. [EC: 6.3.1.10]

Predicted SEED Role

"Adenosylcobinamide-phosphate synthase (EC 6.3.1.10)" (EC 6.3.1.10)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
• adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.10

Sequence Analysis Tools

See A0A160A2H9 at UniProt or InterPro

Protein Sequence (307 amino acids)

>Pf6N2E2_5740 Adenosylcobinamide-phosphate synthase (EC 6.3.1.10) (Pseudomonas fluorescens FW300-N2E2)
VGRAPMSVALLTVAAVALDALLGEPRRWHPLVAFGGFASRIEQRFNSGGRGWRSHGVTAW
IIAVVPLTLLATALSWAPMVGWLVDILALYCALGLRSLGEHVEPVAQALRSGDLDEARLR
VGYLVSRETRELDETAVARAATESVLENGSDAVFAALFWFAVAGAPGVVLYRLSNTLDAM
WGYRNERFERFGWAAAKIDDVLNYIPARLVALTYALLGKTRLALKCWRSQGPTWDSPNAG
PVMAAGAGALGVELGGAAVYHGELHQRPPLGEGVPASADSIDRGWQLVQRGVWLWLLILC
LGAEFYA