Protein Info for Pf6N2E2_5738 in Pseudomonas fluorescens FW300-N2E2
Annotation: Cobyrinic acid A,C-diamide synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to COBB_PSEU5: Hydrogenobyrinate a,c-diamide synthase (cobB) from Pseudomonas stutzeri (strain A1501)
KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 96% identity to pba:PSEBR_a4244)Predicted SEED Role
"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (13/13 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.1.- or 6.3.5.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A161HA96 at UniProt or InterPro
Protein Sequence (438 amino acids)
>Pf6N2E2_5738 Cobyrinic acid A,C-diamide synthase (Pseudomonas fluorescens FW300-N2E2) MTSRHCPAVLIAAPASGQGKTTVTAALARLHRNQGRKVRVFKCGPDFLDPMILERASGAP VYQLDMWMVGEQESRRLLWEAAAEADLILIEGVMGLFDGTPSSADLARHFGVPVLAVIDG TAMAQTFGALALGLARYQADLPFAGVLANRVGTLRHAQLLEGSLTEGLRWYGALSRETGI ELPSRHLGLVQASELNDLDLRLDAAAEALAGSCEVALPPAVEFAAPEVIDPQPWLDGVRI AVARDEAFAFTYGASLDLLRAMGAQLSFFSPIHDSELPEADSLYLPGGYPELHHVALARN TPMLAAIRAHHAAGKPLLAECGGMLYLLDSLTDVEGVRAELLGLLAGEAQMQKRLAALAL QSVDLPEGILRGHTYHHSLTSTELEPIARGHSPNGGRGAEAVFRQGRMTASYVHFYFPSN PRAIAALFAPDLEAAKRP