Protein Info for Pf6N2E2_5732 in Pseudomonas fluorescens FW300-N2E2

Annotation: Chromosomal replication initiator protein DnaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 TIGR03420: DnaA regulatory inactivator Hda" amino acids 5 to 232 (228 residues), 288.2 bits, see alignment E=2e-90 PF00308: Bac_DnaA" amino acids 15 to 212 (198 residues), 72 bits, see alignment E=6.9e-24

Best Hits

KEGG orthology group: K10763, DnaA-homolog protein (inferred from 100% identity to pfl:PFL_4437)

Predicted SEED Role

"Chromosomal replication initiator protein DnaA" in subsystem DNA-replication

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0D9ATG8 at UniProt or InterPro

Protein Sequence (234 amino acids)

>Pf6N2E2_5732 Chromosomal replication initiator protein DnaA (Pseudomonas fluorescens FW300-N2E2)
MKPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKDGVGRTH
LLQAACLRFEQLGEPAVYLPLAELLDRGVEILDNLEQYELVCLDDLQAVAGKADWEEALF
HLFNRLRDSGRRLLIAASTSPRELPVKLADLKSRLTLALIFQMRPLSDEDKLRALQLRAS
RRGLHLTDEVGHFILTRGTRSMSALFELLERLDQASLQAQRKLTIPFLKETLGW