Protein Info for Pf6N2E2_5698 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 963 PF03477: ATP-cone" amino acids 33 to 129 (97 residues), 66.8 bits, see alignment E=3e-22 PF00317: Ribonuc_red_lgN" amino acids 294 to 366 (73 residues), 88.4 bits, see alignment 4e-29 TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit" amino acids 297 to 907 (611 residues), 711.7 bits, see alignment E=3.9e-218 PF02867: Ribonuc_red_lgC" amino acids 371 to 906 (536 residues), 641.5 bits, see alignment E=1.7e-196

Best Hits

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 75% identity to abo:ABO_0711)

Predicted SEED Role

"Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2F2 at UniProt or InterPro

Protein Sequence (963 amino acids)

>Pf6N2E2_5698 Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1) (Pseudomonas fluorescens FW300-N2E2)
MHTDTTRENPQGTAPLAADSNPDLSATAPGQLRVIKRNGTVVPYTDDKITVAITKAFLAV
EGGTAAASSRIHDTVARLTEQVTATFKRRMPSGGTIHIEEIQDQVELALMRAGEQKVARD
YVIYRDSRAKERATRSPADAPVQAHPSIRIARADGSLAPLDMGRLNTIVTEACEGLEEVD
GDLIQRETLKNLYDGVALKDVNTALVMTARTLVEREPNYSFVTARLLMDTLRAEGLSFLE
VAESATHHEMVDLYAKALPAYVAKGIEFELLNPVLAEFDLEKLGKAINHERDQQFTYLGL
QTLYDRYFIHKDGVRFELPQIFFMRVAMGLAIEEKQREDRAIEFYNLLSSFDYMASTPTL
FNAGTLRPQLSSCYLTTVPDDLSGIYGAIHDNAMLSKFAGGLGNDWTPVRALGSYIKGTN
GKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLETWHMDIEEFIELRKNTGDDRRRTHD
MNTANWIPDLFMKRVFDDGKWTLFSPSEVPDLHDLTGKAFEERYEYYEALTEYNKIKLFK
VVQAKDLWRKMLSMLFETGHPWLTFKDPCNLRSPQQHVGVVHSSNLCTEITLNTNKDEIA
VCNLGSINLPNHIVDGKLDTAKLQRTVNTAVRMLDNVIDINYYSVPQAKNSNFKHRPVGL
GIMGFQDALYLQHIPYGSDAAVEFADKSMEAVSYYAIQASCDLADERGAYETFQGSLWSQ
GILPLDSQQILIAQRGQKYIDVDLNESLDWAPVRARVQKGIRNSNIMAIAPTATIANITG
VSQSIEPTYQNLYVKSNLSGEFTVINPYLVRDLKARGLWDSVMINDLKYYDGSVQQIERI
PQELKALYATAFEVDTKWIVDAASRRQKWIDQAQSLNLYIAGASGKKLDVTYRMAWYRGL
KTTYYLRALAATSTEKSTINTGKLNAVSSGGNHGDDSVLAAPAGPAPVPKACAIDEPDCE
ACQ