Protein Info for Pf6N2E2_5674 in Pseudomonas fluorescens FW300-N2E2
Annotation: Low-specificity L-threonine aldolase (EC 4.1.2.48)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to LTAE_ECOLI: Low specificity L-threonine aldolase (ltaE) from Escherichia coli (strain K12)
KEGG orthology group: K01620, threonine aldolase [EC: 4.1.2.5] (inferred from 98% identity to pba:PSEBR_a4311)MetaCyc: 58% identical to low-specificity L-threonine aldolase (Escherichia coli K-12 substr. MG1655)
Threonine aldolase. [EC: 4.1.2.48, 4.1.2.5]; LTAA-RXN [EC: 4.1.2.48, 4.1.2.5, 4.1.2.49]; 4.1.2.48 [EC: 4.1.2.48, 4.1.2.5, 4.1.2.49]; Phenylserine aldolase. [EC: 4.1.2.48, 4.1.2.5, 4.1.2.49, 4.1.2.26]
Predicted SEED Role
"Low-specificity L-threonine aldolase (EC 4.1.2.48)" (EC 4.1.2.48)
MetaCyc Pathways
- L-threonine degradation IV (2/2 steps found)
- glycine biosynthesis IV (1/1 steps found)
- superpathway of L-threonine metabolism (13/18 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.1.2.48, 4.1.2.5
Use Curated BLAST to search for 4.1.2.26 or 4.1.2.48 or 4.1.2.49 or 4.1.2.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A5W3 at UniProt or InterPro
Protein Sequence (334 amino acids)
>Pf6N2E2_5674 Low-specificity L-threonine aldolase (EC 4.1.2.48) (Pseudomonas fluorescens FW300-N2E2) MSVIDLRSDTVTQPSAGMLDAMASAPTGDDVYGEDPTVNRLEAELAARLGFAAALFVPTG TMSNLLGLMAHCERGDEYIVGQQAHTYKYEGGGAAVLGSIQPQPLEVQADGSLDLAQVAA AIKPDDFHFARTRLLALENTMQGKVLPLEYLARARRFTQEHGLALHLDGARLYNAAVKLN VDARQITQHFDSVSVCLSKGLGAPVGSVLCGSVELIGKARRLRKMVGGGMRQAGILAAAG LYALEHQVQRLADDHGNAQRLAEGLRAAGYEVEPVQTNMVYVQMGERAEAIKAFADERGV KLSAAPRLRMVTHMDVSAAQIDQVVATFVEFSRH