Protein Info for Pf6N2E2_5671 in Pseudomonas fluorescens FW300-N2E2

Annotation: Succinylglutamate desuccinylase (EC 3.5.1.96)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03242: succinylglutamate desuccinylase
  • amino acids 6 to 327 (322 residues), 391.7 bits, E=1.3e-121
• PF04952: AstE_AspA
  • amino acids 49 to 328 (280 residues), 271.3 bits, E=5e-85

Best Hits

Swiss-Prot: 92% identical to ASTE_PSEPF: Succinylglutamate desuccinylase (astE) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K05526, succinylglutamate desuccinylase [EC: 3.5.1.96] (inferred from 100% identity to pba:PSEBR_a4313)

Predicted SEED Role

"Succinylglutamate desuccinylase (EC 3.5.1.96)" in subsystem Arginine and Ornithine Degradation (EC 3.5.1.96)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.96

Sequence Analysis Tools

See A0A160A3Y3 at UniProt or InterPro

Protein Sequence (335 amino acids)

>Pf6N2E2_5671 Succinylglutamate desuccinylase (EC 3.5.1.96) (Pseudomonas fluorescens FW300-N2E2)
MLALGKLLELTLAGREPAEKTQLTVEGVRMRWLSEGALEVKPPEARDNGLDLLLSAGIHG
NETAPIELLDRLLHDIARGHLKPRARILFLFGNPEAIRRGERFVEQDVNRLFNGRHELSG
GPEALRACELERLAASFFSRPERSRLHYDLHTAIRGSKIEQFALYPWKEGRQHSRRELAR
LRAGGMEAVLLQNKPSIVFSAYTYDQLGAESFTLELGKARPFGQNDGVNVSLLETRLQQI
IEGNEPELDEGLDGLQLFSVAREIIKHSDSFRLNLPADIENFSELGKGYVLAEDIAQTRW
VIEEEGARIIFPNPKVKNGLRAGILIVPATDENLA