Protein Info for Pf6N2E2_5616 in Pseudomonas fluorescens FW300-N2E2
Updated annotation (from data): outer membrane component of uptake system, probably for ferrous iron
Rationale: PFam PF07433.7 (DUF1513). In a conserved cofit operon with an efeO-like gene (Pf6N2E2_5617) and an efeB-like gene (Pf6N2E2_5618). Another efeO-like gene (Pf6N2E2_2853) is cofit but not nearby.
Original annotation: putative exported protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K09947, hypothetical protein (inferred from 98% identity to pba:PSEBR_a4366)Predicted SEED Role
No annotation
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A3V4 at UniProt or InterPro
Protein Sequence (365 amino acids)
>Pf6N2E2_5616 outer membrane component of uptake system, probably for ferrous iron (Pseudomonas fluorescens FW300-N2E2) MFRRQVLALGSLLLGAVTLGGWTLFKQKDKHPLLLSARDDGDGKHYAVGYRLDGTRVFAT QVGQRCHDIINHPTQPIALFVARRPGTESYLIDLRDGALLQTIASLPNRHFYGHAVIHNS GEWLYATENDTTDPGRGLLGVYRFEGERLVHSGEISTHGIGPHQVSWMPDGETLVVANGG IRTEAESRVEMNLDAMEPSLVLMQRDGTLLSKETLAQSMNSVRHLGIASDGTIVSGQQFM GAAHESSELLAIKRPGQPFQAFPLPEEQLQAMGHYTASVAVHSDLRLVALTAPRGNRFFI WDLDSGEVRLDAPLPDCAGVGAVADGFVVTSGQGRCRYYDCRQTQLVAKPLDLPAGLWDN HLHLA