Protein Info for Pf6N2E2_5614 in Pseudomonas fluorescens FW300-N2E2

Annotation: RND multidrug efflux transporter; Acriflavin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1008 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 335 to 353 (19 residues), see Phobius details amino acids 359 to 379 (21 residues), see Phobius details amino acids 385 to 409 (25 residues), see Phobius details amino acids 430 to 450 (21 residues), see Phobius details amino acids 462 to 485 (24 residues), see Phobius details amino acids 525 to 544 (20 residues), see Phobius details amino acids 845 to 865 (21 residues), see Phobius details amino acids 872 to 892 (21 residues), see Phobius details amino acids 899 to 921 (23 residues), see Phobius details amino acids 948 to 970 (23 residues), see Phobius details amino acids 978 to 1002 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 6 to 1002 (997 residues), 922 bits, see alignment E=4.3e-281 PF03176: MMPL" amino acids 294 to 490 (197 residues), 35.6 bits, see alignment E=5.4e-13 amino acids 811 to 1003 (193 residues), 28.6 bits, see alignment E=7.3e-11

Best Hits

Swiss-Prot: 45% identical to Y895_HAEIN: Uncharacterized transporter HI_0895 (HI_0895) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a4368)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A620 at UniProt or InterPro

Protein Sequence (1008 amino acids)

>Pf6N2E2_5614 RND multidrug efflux transporter; Acriflavin resistance protein (Pseudomonas fluorescens FW300-N2E2)
MAFTDPFIRRPVLATVVSLLIVLLGFQAWSKLPLRQYPQMENALITVTTAYPGANAETIQ
GYITQPMQQSLASAEGIDYMTSVSRQNFSVISVYARIGSNSDRLFTELLAKANEVKNQLP
QDAEDPVLSREAADASALMYISFFSKELNNPQITDYLSRVIQPKLATLPGMAEAEILGNQ
VFAMRLWLDPVKLAGFGLTAADVTNAVRQHNFLSAAGEVKGEYIVTSINANTELKSAEAF
AAIPLKTDGDSRVLLRDVARVEMGAENYDTISSFGGTPSVYIGIKATPGANPLDVIKEVR
KIMPDMEAQLPANLKAEIAYDATLFIQASIDEVVKTLFEAVLIVIVVVFLFLGALRSVVI
PVVTIPLSMIGVMFFMQLMGYSMNLLTLLAMVLAIGLVVDDAIVVVENIHRHIEEGKTPF
DAAIEGAREIAMPVVSMTITLAAVYAPIGLLQGLTGALFKEFALTLAGAVVISGIVALTL
SPMMCAMLLRHDENPSGLAHRLDMVFERLKSRYQRMLHGTLNTRPVVLVFAVIVLLLIPV
LIMFTKSELAPDEDQGIIFMMANAPQPTNLDYLNTYTDHFITIFKEFPEYYSSFQINGYN
GVQSGIGGFLLKPWNERSRTQMEILPEVQAKLESVPGLQIFGFNLPSLPGTGEGLPFAFV
INSPKDYATLLEIAERVKKRALESGKFAFMDIDLAFDKPEVVVDIDRAKAAQMGVSMQDL
GGTLATLLGEAEINRFTLEGRSYKVIAQVERPFRDNPDWLNNYYVKNAKGESLPLSTLIK
VSDRARPRQLNQFQQLNAVTISGFPVVSMGEAIETVRQIAREEAPAGFAFDYAGASRQFV
QEGSALWVTFALALAIIFLVLAAQFESFRDPLVILVTVPLSICGALIPLFLGWSSMNIYT
QVGLVTLIGLISKHGILIVEFANQLRREQNLTPREAVEQAASIRLRPVLMTTAAMVFGMV
PLIFATGAGAVSRFDIGTVIATGMSIGTLFTLFVLPCVYTLLAKPDKP