Protein Info for Pf6N2E2_5581 in Pseudomonas fluorescens FW300-N2E2

Annotation: Formate dehydrogenase-O, major subunit (EC 1.2.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 PF00384: Molybdopterin" amino acids 65 to 482 (418 residues), 117.8 bits, see alignment E=5.7e-38 PF01568: Molydop_binding" amino acids 576 to 695 (120 residues), 79.2 bits, see alignment E=2.4e-26

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a4398)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GT84 at UniProt or InterPro

Protein Sequence (702 amino acids)

>Pf6N2E2_5581 Formate dehydrogenase-O, major subunit (EC 1.2.1.2) (Pseudomonas fluorescens FW300-N2E2)
MTKTLHHRACHLCEAICGLTLETTETDGQGLAITSIKGDALDTFSRGHICPKAVALQDIQ
NDPDRLRQPMQRVGNEWQPIAWDDAFALVAERLADIQARHGQNAVAVYQGNPSVHNYGLM
THSNYFLGLLKTRSRFSATSVDQLPHHLTSYLMYGHGLLLPIPDIDHTDFMLILGGNPLA
SNGSIMTVPDVEKRLKAIQARGGKVVVVDPRRSETAAIANQHVFVRPGGDAALLSGLLNT
LFSEGLTRDSHLPVDGLDEVREAVATFTAEAMSPLCAVPAAQIRQLARDFAAAPSAVCYG
RMGVSTQAFGTLCHWLIQVINLVTGNLDRVGGALCTEPAVDLVATTSGGHFNRWQSRVSG
RPEYSGELPVSALAEEMLTEGEGQIRALVTVAGNPVLSTPNGRQLEQALDGLEFMVSVDL
YINETTRYADLILPSTSALENDHYDTTFNLFAVRNVTRFNRAILPKPEGALHDWEIFVGL
AKAFAARTGKELKPTMPPAQMIDFGLRSGAYGDASPHKLSLATLFDHPHGIDLGALKPNL
AARLKTENQRVQAAPAVILVDLERFAGLQAPKPDELLMIGRRHVRSNNSWMHNFHRLVKG
KPRHQLLMHPDDLASRGLVDGQRVTVSSRVGVIEVEVLGCLDMMKGVVSLPHGWGHARPG
VQMTIASGQPGSSANDLTDDCQLDELSGNAALNGVPVKVAAA