Protein Info for Pf6N2E2_5417 in Pseudomonas fluorescens FW300-N2E2

Annotation: Valyl-tRNA synthetase (EC 6.1.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 transmembrane" amino acids 501 to 523 (23 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 928 (928 residues), 1200.6 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 628 (615 residues), 510 bits, see alignment E=1.2e-156 PF09334: tRNA-synt_1g" amino acids 38 to 88 (51 residues), 21.8 bits, see alignment 1.8e-08 PF13603: tRNA-synt_1_2" amino acids 244 to 341 (98 residues), 28.6 bits, see alignment E=2.4e-10 PF08264: Anticodon_1" amino acids 669 to 821 (153 residues), 150.1 bits, see alignment E=1.1e-47 PF10458: Val_tRNA-synt_C" amino acids 883 to 945 (63 residues), 65.2 bits, see alignment 1.4e-21

Best Hits

Swiss-Prot: 66% identical to SYV_COLP3: Valine--tRNA ligase (valS) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

MetaCyc: 64% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3N1 at UniProt or InterPro

Protein Sequence (948 amino acids)

>Pf6N2E2_5417 Valyl-tRNA synthetase (EC 6.1.1.9) (Pseudomonas fluorescens FW300-N2E2)
MDKTYQPHAIETSWYNTWESENYFAPQGAGDSYTIMIPPPNVTGSLHMGHGFNNAIMDAL
IRFRRMQGRNTLWQPGTDHAGIATQMLVERRLEAQGQNRHDLGREKFLEKVWEWKDESGG
NISRQIRRLGSSVDWSRERFTMDDGLSEAVKEAFVRLHEDGLIYRGKRLVNWDTKLHTAI
SDLEVENHDEKGFLWNLKYPLADGAKTAEGKDYLIVATTRPETMLGDSAVAVNPNDERYQ
ALIGKFVELPLVGRRIPIIGDDYCDPEFGTGCVKITPAHDFNDYEVGKRHNLPLLNIFDK
NANVLPAAQVFNLDGTLNESIDGQIPAEYAGLDRFEARKQIVAAFDAAGLLVSVDDHALK
VPKGDRSGTIIEPWLTDQWYVSTKPLAEPAIAAVEDGRIQFVPKQYENMYFSWMRDIQDW
CISRQLWWGHRIPAWYDESGKVYVGRDEAEVRAKHNLGPDVALAQDNDVLDTWFSSGLWT
FSTLGWPQQTEFLKKFHSTDVLVTGFDIIFFWVARMIMLTMHLVKNEDGTPQVPFKTVYV
HGLVRDGQGQKMSKSKGNVLDPLDIIDGIDLETLVQKRTSGLMQPKLAKKIEKATREEFA
EGIASYGTDALRFTFCSLASTGRDIKFDMGRVEGYRNFCNKIWNAARYVLDKGEDCGQNG
EAFELTLADRWIISQLQRTEAEVTRQLDQFRFDLAAQALYEFIWNQYCDWYLELSKPVLW
DENAPVERQRGTRRTLVRVLEVALRLAHPFMPFITEEIWQRIAPLAGAQGKTIMLQPWPV
ANESRIDPAAEDDIEWLKTFMLGLRNIRAEMNIGPGKPLTLFLKNASAEDLRRLNENEAL
LKKLAKLESVTVLAAGEEAPLSATALVGEMEVLVPMAGLIDKAAELARLDKEILRLKGEV
QRVGGKLSNAGFVDKAPAEVIEKERAKLAEAEQALGKLAEQHARISSL