Protein Info for Pf6N2E2_5365 in Pseudomonas fluorescens FW300-N2E2

Annotation: Heavy metal sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 transmembrane" amino acids 7 to 31 (25 residues), see Phobius details amino acids 159 to 182 (24 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 6 to 454 (449 residues), 442.5 bits, see alignment E=9.8e-137 PF00512: HisKA" amino acids 239 to 303 (65 residues), 53.1 bits, see alignment E=4.1e-18 PF02518: HATPase_c" amino acids 349 to 454 (106 residues), 83.8 bits, see alignment E=1.7e-27 PF14501: HATPase_c_5" amino acids 351 to 449 (99 residues), 22.3 bits, see alignment E=1.5e-08

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 98% identity to pba:PSEBR_a4613)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3Q4 at UniProt or InterPro

Protein Sequence (455 amino acids)

>Pf6N2E2_5365 Heavy metal sensor histidine kinase (Pseudomonas fluorescens FW300-N2E2)
MSVPDSIALRLSGMFTLVAALVFLLIGGALYQQVGKGLGLLPEAELDARYSVLESTVGRY
GTPEHWVKINNKLRLLGEEDKRIHFWIVSDDPRFEYGAPDAQIRAFAQGALGKRDLTLPH
RDYPMKVLVSQFPAKDQRPPLRFMIGIDTQTFYQTQHRLLVALVSLAIVGVLLASLLGYW
VARIGLKPLIKLSDEARRLAPPRLSGRLQLSPLPPELSQFVSSFNATLDRVEQAYSRLES
FNADVAHELRSPLTNLIGQTQVALTRGRSAEHYFEVLQSNLEELERLRSIINDMLFLASA
DQGSKATKLTSASLAGEVATTLDYLDFILEDAQVQVRVSGDAQANIEIAHLRRALINLLS
NAVQHTAPGQVIDVHIEALGDQITLAVTNPGEPIAGEHLPRLFERFYRVDASRSNSGANH
GLGLAIVKAIALMHGGDVFVRSDQGANTFGIRLPA