Protein Info for Pf6N2E2_533 in Pseudomonas fluorescens FW300-N2E2

Annotation: Na(+) H(+) antiporter subunit G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 117 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 45 to 65 (21 residues), see Phobius details amino acids 72 to 93 (22 residues), see Phobius details TIGR01300: monovalent cation/proton antiporter, MnhG/PhaG subunit" amino acids 15 to 101 (87 residues), 68.6 bits, see alignment E=2.3e-23 PF03334: PhaG_MnhG_YufB" amino acids 16 to 96 (81 residues), 73.3 bits, see alignment E=7.7e-25

Best Hits

Swiss-Prot: 39% identical to PHAG_RHIME: Probable K(+)/H(+) antiporter subunit G (phaG) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K05564, multicomponent K+:H+ antiporter subunit G (inferred from 97% identity to pba:PSEBR_a3338)

Predicted SEED Role

"Na(+) H(+) antiporter subunit G" in subsystem Sodium Hydrogen Antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZRE8 at UniProt or InterPro

Protein Sequence (117 amino acids)

>Pf6N2E2_533 Na(+) H(+) antiporter subunit G (Pseudomonas fluorescens FW300-N2E2)
MTGELSMWVEIPVAILLVLSGLFALIGAIGLVRLKDYFQRMHPPALASTLGAWCVALASI
VYFSALKSGPVLHAWLIPILLSITVPVTTLLLARAALFRKRMAGDDVPAEVSSRRND