Protein Info for Pf6N2E2_5321 in Pseudomonas fluorescens FW300-N2E2

Annotation: Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 36 to 59 (24 residues), see Phobius details amino acids 80 to 103 (24 residues), see Phobius details amino acids 109 to 128 (20 residues), see Phobius details amino acids 148 to 172 (25 residues), see Phobius details amino acids 178 to 197 (20 residues), see Phobius details amino acids 209 to 227 (19 residues), see Phobius details amino acids 233 to 252 (20 residues), see Phobius details amino acids 264 to 281 (18 residues), see Phobius details TIGR01473: protoheme IX farnesyltransferase" amino acids 4 to 282 (279 residues), 303.7 bits, see alignment E=8.2e-95 PF01040: UbiA" amino acids 19 to 268 (250 residues), 200.2 bits, see alignment E=1.8e-63

Best Hits

Swiss-Prot: 95% identical to CYOE2_PSEPF: Protoheme IX farnesyltransferase 2 (cyoE2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K02301, protoheme IX farnesyltransferase [EC: 2.5.1.-] (inferred from 99% identity to pba:PSEBR_a4669)

MetaCyc: 66% identical to heme O synthase (Escherichia coli K-12 substr. MG1655)
HEMEOSYN-RXN [EC: 2.5.1.141]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.- or 2.5.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1P1 at UniProt or InterPro

Protein Sequence (295 amino acids)

>Pf6N2E2_5321 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB (Pseudomonas fluorescens FW300-N2E2)
MSLKHFIQITKPGIIFGNVLSVAGGFFLASKGHVDLAIFLAAMIGTSLVVASGCVFNNCI
DRDIDIKMERTKNRALVQGLIPVQLALAFATVLGVAGVALLYWVANPLAALFAVIGFVIY
VGLYSLYLKRKSVHGTLVGSLSGAMPPVIGYVAVSNSFDMAALTLLVMFSLWQMPHSYAI
AIFRFNDYLAASIPVLPVKRGILVAKKHILLYILAFLVATLMLTFSGYAGMSYLAVAAAM
GMYWLYMAWTGYKAVDDTVWARKLFVFSIFTITALSVMMSLDFKVPSELLLTYAP