Protein Info for Pf6N2E2_5319 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02842: cytochrome o ubiquinol oxidase, subunit III
  • amino acids 29 to 207 (179 residues), 282.3 bits, E=1.1e-88
• PF00510: COX3
  • amino acids 29 to 206 (178 residues), 65.8 bits, E=2.9e-22

Best Hits

Swiss-Prot: 80% identical to CYOC_PSEPU: Cytochrome bo(3) ubiquinol oxidase subunit 3 (cyoC) from Pseudomonas putida

KEGG orthology group: K02299, cytochrome o ubiquinol oxidase subunit III [EC: 1.10.3.-] (inferred from 98% identity to pba:PSEBR_a4671)

MetaCyc: 80% identical to cytochrome bo terminal oxidase subunit III (Pseudomonas putida KT2440)
RXN0-5268 [EC: 7.1.1.3]

Predicted SEED Role

"Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• NADH to cytochrome bo oxidase electron transfer II (2/2 steps found)
• glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
• proline to cytochrome bo oxidase electron transfer (2/2 steps found)
• succinate to cytochrome bo oxidase electron transfer (2/2 steps found)
• D-lactate to cytochrome bo oxidase electron transfer (1/2 steps found)
• pyruvate to cytochrome bo oxidase electron transfer (1/2 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.3

Sequence Analysis Tools

See A0A165ZP01 at UniProt or InterPro

Protein Sequence (208 amino acids)

>Pf6N2E2_5319 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) (Pseudomonas fluorescens FW300-N2E2)
MSNLVTTVGHAHGHDHGHDDHHHDSGEMTVFGFWLYLMTDCILFASIFAAYAVLVNNVAG
GPSGHDIFELPYVLGETALLLFSSITYGFAMLALFKGKKTQVLGWLAMTFLFGAGFIGME
INEFHILISEGFGPSRSGFLSGFFTLVGTHGLHVTSGLIWMAIMMYQVHKNGLTATNKTR
LSCLSLFWHFLDVVWICVFTVVYLMGTL