Protein Info for Pf6N2E2_5299 in Pseudomonas fluorescens FW300-N2E2

Annotation: Benzoate transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 47 to 65 (19 residues), see Phobius details amino acids 72 to 90 (19 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 122 to 140 (19 residues), see Phobius details amino acids 146 to 163 (18 residues), see Phobius details amino acids 170 to 188 (19 residues), see Phobius details amino acids 210 to 231 (22 residues), see Phobius details amino acids 249 to 264 (16 residues), see Phobius details amino acids 290 to 315 (26 residues), see Phobius details amino acids 321 to 343 (23 residues), see Phobius details amino acids 352 to 383 (32 residues), see Phobius details PF03594: BenE" amino acids 7 to 383 (377 residues), 521.3 bits, see alignment E=7e-161 TIGR00843: benzoate transporter" amino acids 10 to 384 (375 residues), 480.6 bits, see alignment E=1.8e-148

Best Hits

Swiss-Prot: 53% identical to BENE_ACIAD: Benzoate membrane transport protein (benE) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05782, benzoate membrane transport protein (inferred from 99% identity to pba:PSEBR_a4687)

Predicted SEED Role

"Benzoate transport protein" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZNY5 at UniProt or InterPro

Protein Sequence (401 amino acids)

>Pf6N2E2_5299 Benzoate transport protein (Pseudomonas fluorescens FW300-N2E2)
MDTFRKDLSLSAIIAGFIAVIISYAGPLIIVFQAAKEAHLPNDVVSSWIWAISIGSGITG
LFLSWRLRVPVITAWSTPGAALLVSMLPTVTLPQAIGAYVVASMIIALVGLSGAFDKLMS
RLPKAIAAAMLAGILFRFGAELFTSIKLQPALVLAMIAAYLIFKRFSPRYAILSVLIVGC
AVAASFGELNSTSITIAVAQPVFIAPEWNWHAIINIGLPLALVTLTGQYVPGMAVLRTSG
YNTPARSIISVTAIGSILMAPFGSHGLNLAAITAAICTGREAHEDRDKRYMAGIACGVFY
ILMGTFGATLASVFSALPKELIASLAGLALFGAISAGLTGAMADEKQREAALITFLVTAS
GMSFLGLAAAFWGLIFGLVAHFVLTYTRERKAAVIAEGSRP