Protein Info for Pf6N2E2_5291 in Pseudomonas fluorescens FW300-N2E2

Annotation: Fumarylacetoacetase (EC 3.7.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR01266: fumarylacetoacetase" amino acids 9 to 433 (425 residues), 629.4 bits, see alignment E=1.4e-193 PF09298: FAA_hydrolase_N" amino acids 23 to 126 (104 residues), 75.8 bits, see alignment E=2.8e-25 PF01557: FAA_hydrolase" amino acids 140 to 429 (290 residues), 126.4 bits, see alignment E=1.4e-40

Best Hits

Swiss-Prot: 49% identical to FAH_ORYSJ: Fumarylacetoacetase (FAH) from Oryza sativa subsp. japonica

KEGG orthology group: K01555, fumarylacetoacetase [EC: 3.7.1.2] (inferred from 99% identity to pba:PSEBR_a4695)

Predicted SEED Role

"Fumarylacetoacetase (EC 3.7.1.2)" in subsystem Homogentisate pathway of aromatic compound degradation or Salicylate and gentisate catabolism (EC 3.7.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1L9 at UniProt or InterPro

Protein Sequence (434 amino acids)

>Pf6N2E2_5291 Fumarylacetoacetase (EC 3.7.1.2) (Pseudomonas fluorescens FW300-N2E2)
MTQLSPARSWVACANGHADFPLQNLPLGIFSVDGGALRSGVAIGERIFDLQAALHAGLFD
GAARQAVEAMADGQLNGFFDLGRGARVALRERLLELLREDSPLRGEVEAQGAKLLLLAAD
CQMHLPARINDYTDFYVGIEHAQNVGKLFRPDNPLLPNYKYVPIGYHGRASTIRPSGTDV
RRPKGQTLPAGQTEPTFGPCARLDYELELGIWIGKGNALGEPIPIGDAAEHIGGFCLLND
WSARDIQAWEYQPLGPFLSKSFLTSISPWVVTAEALEPFRRPQPARPQGDPQPLPYLLDK
RDQAGGAFDIELEVLLLTEAMGEQNLPAQRLTLSNTQYMYWTVAQMVAHHSVNGCQLQAG
DLFGSGTLSGPENHQFGSLLEITEGGKKPIELASGEVRRFLEDGDEIILRARCRREGFAS
IGFGECRGKILPAS