Protein Info for Pf6N2E2_5281 in Pseudomonas fluorescens FW300-N2E2

Annotation: Alpha/beta hydrolase fold (EC 3.8.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00561: Abhydrolase_1
  • amino acids 35 to 275 (241 residues), 75.9 bits, E=4.1e-25
• PF12697: Abhydrolase_6
  • amino acids 36 to 279 (244 residues), 66.8 bits, E=4.6e-22

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a4704)

Predicted SEED Role

"Alpha/beta hydrolase fold (EC 3.8.1.5)" (EC 3.8.1.5)

MetaCyc Pathways

• 1,2-dichloroethane degradation (2/4 steps found)
• γ-hexachlorocyclohexane degradation (6/11 steps found)

KEGG Metabolic Maps

• 1,2-Dichloroethane degradation
• 3-Chloroacrylic acid degradation
• Naphthalene and anthracene degradation
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 3.8.1.5

Use Curated BLAST to search for 3.8.1.5

Sequence Analysis Tools

See A0A160A1K6 at UniProt or InterPro

Protein Sequence (300 amino acids)

>Pf6N2E2_5281 Alpha/beta hydrolase fold (EC 3.8.1.5) (Pseudomonas fluorescens FW300-N2E2)
MPLAQIPLRIWRQRSQIFMFRGHAIRYWVAGQGEPLLLIHGFPTASWDWHYLWQPLAQRY
RVIACDMLGFGDSAKPTDHVYSLLEQADLQQALLAHLNVRGALHVLAHDYGDSVAQELLA
RHYEARIHLASCVFLNGGLFPETHRPVWAQKLLLSPLGWLLGRAFSRESLVSSFRQIFGP
RTTPTESELDDFWSLVEAHRGPRIMHKLIAYIPERRVQRDRWVQAMQRGDVPLRVIDGAV
DPISGEHMVARYEALIPSPDTVLLPDIGHYPHTEAPVQVLKHYLAFREAIAAPHRQMACS