Protein Info for Pf6N2E2_5263 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glutathione peroxidase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 160 PF00255: GSHPx" amino acids 4 to 110 (107 residues), 134.1 bits, see alignment E=2.3e-43 PF00578: AhpC-TSA" amino acids 4 to 92 (89 residues), 27.3 bits, see alignment E=4.6e-10 PF08534: Redoxin" amino acids 5 to 65 (61 residues), 28.9 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 55% identical to GPX2_SYNY3: Hydroperoxy fatty acid reductase gpx2 (gpx2) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00432, glutathione peroxidase [EC: 1.11.1.9] (inferred from 94% identity to pfo:Pfl01_0883)

MetaCyc: 55% identical to hydroperoxy fatty acid reductase 2 (Synechocystis sp. PCC 6803)
RXN-13944 [EC: 1.11.1.22]; 1.11.1.- [EC: 1.11.1.22]

Predicted SEED Role

"Glutathione peroxidase family protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.11.1.9

Use Curated BLAST to search for 1.11.1.22 or 1.11.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A3G6 at UniProt or InterPro

Protein Sequence (160 amino acids)

>Pf6N2E2_5263 Glutathione peroxidase family protein (Pseudomonas fluorescens FW300-N2E2)
MSAFHDLKLKALDGQELALAPFKGQVVLVVNVASKCGLTPQYAALENLYQQYKAKGFSVL
GLPCNQFAGQEPGTEQEIQDFCSLNYGVTFPLSSKLDVNGHDRHQLYRLLAGEGAEFPGD
ITWNFEKFLLGKDGRVLARFSPRTAPDDPTVIQAIEKALG