Protein Info for Pf6N2E2_5242 in Pseudomonas fluorescens FW300-N2E2

Annotation: Malate:quinone oxidoreductase (EC 1.1.5.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 6 to 491 (486 residues), 756.4 bits, E=1.8e-231
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 7 to 485 (479 residues), 794.1 bits, E=2.3e-243
• PF01266: DAO
  • amino acids 8 to 278 (271 residues), 35.8 bits, E=1.1e-12

Best Hits

Swiss-Prot: 96% identical to MQO_PSEFL: Probable malate:quinone oxidoreductase (mqo) from Pseudomonas fluorescens

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 99% identity to pba:PSEBR_a4738)

MetaCyc: 53% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Sequence Analysis Tools

See A0A160A4D1 at UniProt or InterPro

Protein Sequence (501 amino acids)

>Pf6N2E2_5242 Malate:quinone oxidoreductase (EC 1.1.5.4) (Pseudomonas fluorescens FW300-N2E2)
MAHNEAVDVVLVGAGIMSATLAVLLKELDPAISLEVVELMDSGAAESSNPWNNAGTGHAG
LCELNYTPQAADGNVDIKKAVHINTQFEVSKQFWTYLTKKGTFGSSKSFIAPVPHLSFVQ
GEKGVSFLKKRFELMRQHHAFADMEYTEDKARMAEWMPLMMPGRPADEVIAATRVMNGTD
VNFGALTNQLLKHLTSAPDTQVKYCKRVTGLKRNGSGWTVSIKDVNSGSSRDVDAKFVFL
GAGGAALPLLQASGIEESKGFGGFPVSGQWLRCDNPEVVKHHQAKVYSQAAVGSPPMSVP
HLDTRVVDGKKSLLFGPYAGFTTKFLKHGSIMDLPLSVRAGNIGPMLAVARDNMDLTKYL
ISEVMQSMEQRLESLRRFYPEAKAEDWRLEVAGQRVQIIKKDPKKGGVLQFGTELVAAKD
GSLAALLGASPGASVTVSIMLELIERCFPDKAKGEWAAKLAEIFPAREKVLETDAALYRK
INAQNNIALELVEDSKAQSYA