Protein Info for Pf6N2E2_5235 in Pseudomonas fluorescens FW300-N2E2
Annotation: Uracil phosphoribosyltransferase (EC 2.4.2.9)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to UPP_PSEF5: Uracil phosphoribosyltransferase (upp) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
KEGG orthology group: K00761, uracil phosphoribosyltransferase [EC: 2.4.2.9] (inferred from 100% identity to pba:PSEBR_a4744)MetaCyc: 69% identical to uracil phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Uracil phosphoribosyltransferase. [EC: 2.4.2.9]
Predicted SEED Role
"Uracil phosphoribosyltransferase (EC 2.4.2.9)" in subsystem De Novo Pyrimidine Synthesis or LMPTP YwlE cluster (EC 2.4.2.9)
MetaCyc Pathways
- superpathway of pyrimidine nucleobases salvage (4/4 steps found)
- pyrimidine nucleobases salvage II (2/2 steps found)
- pyrimidine nucleobases salvage I (1/1 steps found)
- superpathway of pyrimidine ribonucleosides salvage (6/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.4.2.9
Use Curated BLAST to search for 2.4.2.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A397 at UniProt or InterPro
Protein Sequence (212 amino acids)
>Pf6N2E2_5235 Uracil phosphoribosyltransferase (EC 2.4.2.9) (Pseudomonas fluorescens FW300-N2E2) MPILEIRHPLIRHKLGLMRRADISTKNFRELAQEVGALLTYEATKDLPLESYEIDGWAGT VQVEKIAGKKITVVPILRAGIGMLEGVLSLIPGAKVSAVGVARNEQTLQAHTYLEKLVPE IDERLAMIIDPMLATGSSMVATIDLLKKAGCRDIRAMVLVAAPEGIKAVQDAHPDVTIYT ASIDQKLNEHGYIIPGLGDAGDKIFGTKQKDA