Protein Info for Pf6N2E2_5216 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF13793: Pribosyltran_N" amino acids 4 to 121 (118 residues), 172.6 bits, see alignment E=3.6e-55 TIGR01251: ribose-phosphate diphosphokinase" amino acids 4 to 313 (310 residues), 406.9 bits, see alignment E=2.2e-126 PF00156: Pribosyltran" amino acids 158 to 254 (97 residues), 72.4 bits, see alignment E=4.2e-24 PF14572: Pribosyl_synth" amino acids 202 to 313 (112 residues), 118.6 bits, see alignment E=4.9e-38

Best Hits

Swiss-Prot: 98% identical to KPRS_PSESM: Ribose-phosphate pyrophosphokinase (prs) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00948, ribose-phosphate pyrophosphokinase [EC: 2.7.6.1] (inferred from 98% identity to pen:PSEEN0857)

MetaCyc: 67% identical to ribose-phosphate diphosphokinase (Escherichia coli K-12 substr. MG1655)
Ribose-phosphate diphosphokinase. [EC: 2.7.6.1]

Predicted SEED Role

"Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)" in subsystem De Novo Purine Biosynthesis or Pentose phosphate pathway (EC 2.7.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.1

Use Curated BLAST to search for 2.7.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZNQ7 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Pf6N2E2_5216 Ribose-phosphate pyrophosphokinase (EC 2.7.6.1) (Pseudomonas fluorescens FW300-N2E2)
VSKMMVFTGNANPDLARRVVRQLHIPLGDISVGKFSDGEITAEINENVRGKDVFIIQPTC
APTNDNLMELVVMADAFRRSSATRITAVIPYFGYARQDRRPRSARVAISAKVVADMLTVV
GIDRVLTVDLHADQIQGFFDIPVDNIYGSPVLVDDIEDQRFENLMIVSPDIGGVVRARAV
AKSLGVDLGIIDKRREKANHSEVMHIIGDVEGRTCILVDDMVDTAGTLCHAAKALKEHGA
AKVFAYCTHPVLSGRAIENIENSVLDELVVTNTIPLSAAAQACVRIRQLDIAPVVAEAVR
RISNEESISAMFR