Protein Info for Pf6N2E2_5205 in Pseudomonas fluorescens FW300-N2E2

Annotation: Permease of the drug/metabolite transporter (DMT) superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details amino acids 48 to 66 (19 residues), see Phobius details amino acids 86 to 110 (25 residues), see Phobius details amino acids 130 to 148 (19 residues), see Phobius details amino acids 154 to 173 (20 residues), see Phobius details amino acids 192 to 209 (18 residues), see Phobius details amino acids 215 to 234 (20 residues), see Phobius details amino acids 245 to 264 (20 residues), see Phobius details amino acids 270 to 289 (20 residues), see Phobius details PF00892: EamA" amino acids 17 to 145 (129 residues), 38.7 bits, see alignment E=5.4e-14 amino acids 157 to 286 (130 residues), 54.4 bits, see alignment E=7.8e-19

Best Hits

Swiss-Prot: 49% identical to Y1977_PSEAE: Uncharacterized protein PA1977 (PA1977) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 67% identity to ppu:PP_3521)

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A5B0 at UniProt or InterPro

Protein Sequence (295 amino acids)

>Pf6N2E2_5205 Permease of the drug/metabolite transporter (DMT) superfamily (Pseudomonas fluorescens FW300-N2E2)
MTAITDPDRNETSRSIWALQAILCLVAFAANSIFCRQALMNGQIDPDSFTAIRLLSGGVF
LLLLIWRRNPRPSIGGSWRGGLSLFLYAYLFSIAYVQLGAGVGALILFGAVQITMFIRSW
QEGEQIQKKVLIGMITAFCGLLAMLLPGADAPPIGSALLMAMSGVSWGIYSVLGKRSANP
LADTTGNFARSLPLLFLLGPGMAFGSGAHMSSPGVLYALASGVLASGCGYALWYGTVKQI
SSQQAATLQLSVPIIAALGGVWLLSEPMSIRLLMVSAVVLGGIALALPANRHHGN