Protein Info for Pf6N2E2_5143 in Pseudomonas fluorescens FW300-N2E2

Annotation: Type IV pilin PilA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 139 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details PF07963: N_methyl" amino acids 1 to 27 (27 residues), 40.6 bits, see alignment 1.2e-14 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 4 to 27 (24 residues), 36.3 bits, see alignment 1.6e-13 PF00114: Pilin" amino acids 35 to 134 (100 residues), 82.4 bits, see alignment E=4.4e-27

Best Hits

Swiss-Prot: 53% identical to FMWC_PSEPU: Fimbrial protein (pilA) from Pseudomonas putida

KEGG orthology group: K02650, type IV pilus assembly protein PilA (inferred from 98% identity to pba:PSEBR_a4825)

Predicted SEED Role

"Type IV pilin PilA" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H4P9 at UniProt or InterPro

Protein Sequence (139 amino acids)

>Pf6N2E2_5143 Type IV pilin PilA (Pseudomonas fluorescens FW300-N2E2)
MNKQSGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLAEVSALKVPFEDVMNQG
TNPSLANIGGTSPTSNCTLTASGTAADGAGTIACTLLNAPAPVLGKAITLTRSTQGVWTC
GSTAAQEFLPAGCTGAANP