Protein Info for Pf6N2E2_5120 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glycine oxidase ThiO (EC 1.4.3.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 signal peptide" amino acids 1 to 6 (6 residues), see Phobius details amino acids 25 to 25 (1 residues), see Phobius details transmembrane" amino acids 7 to 24 (18 residues), see Phobius details PF00890: FAD_binding_2" amino acids 7 to 214 (208 residues), 40.7 bits, see alignment E=3.6e-14 PF01266: DAO" amino acids 7 to 347 (341 residues), 245.3 bits, see alignment E=2.8e-76 TIGR02352: glycine oxidase ThiO" amino acids 7 to 351 (345 residues), 267.8 bits, see alignment E=7.2e-84

Best Hits

Swiss-Prot: 78% identical to GLYOX_PSEPK: Glycine oxidase (thiO) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03153, glycine oxidase [EC: 1.4.3.19] (inferred from 94% identity to pba:PSEBR_a4846)

Predicted SEED Role

"Glycine oxidase ThiO (EC 1.4.3.19)" in subsystem Thiamin biosynthesis (EC 1.4.3.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.19

Use Curated BLAST to search for 1.4.3.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A463 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Pf6N2E2_5120 Glycine oxidase ThiO (EC 1.4.3.19) (Pseudomonas fluorescens FW300-N2E2)
MTRQQQVVIVGGGVIGLLTAFNLASEGQRVVLLDRSSLGQESSWAGGGIVSPLYPWRYSP
AVTALAHWSQDFYPQLGERLFAATGIDPQVHVTGLYWLDLDDQDEALAWAEREGRPLRAV
DISAVHDAVPVLGPGFSRAIYMADVANVRNPRLVKSLKAALLALPNVTVHEHCEVSGFIR
EGATVIGVDSSAGPIRGDQVVLAAGAWSGELLKTLGLKLPVEPVKGQMILYKCASDFLSS
MVLAKGRYAIPRRDGHILVGSTLEREGFDKTPTDAALDSLKASAQQLIPALTGAEVVGHW
AGLRPGSPEGIPYIGEVPGVGGLWLNCGHYRNGLVLAPASCRLFTDLMLGREAVIDPAPY
APAGRL