Protein Info for Pf6N2E2_512 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29)
Rationale: Specifically important in carbon source m-Inositol. This protein is a fusion of 5-keto-deoxygluconate kinase and DUF2090. As DUF2090 has distant similarity to aldolases, it may provide the missing 5-dehydro-2-deoxyphosphogluconate aldolase activity.
Original annotation: 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 TIGR04382: 5-dehydro-2-deoxygluconokinase" amino acids 11 to 335 (325 residues), 404 bits, see alignment E=2.1e-125 PF00294: PfkB" amino acids 13 to 328 (316 residues), 151.5 bits, see alignment E=3.5e-48 PF09863: DUF2090" amino acids 331 to 643 (313 residues), 441.8 bits, see alignment E=1.2e-136

Best Hits

KEGG orthology group: K03338, 5-dehydro-2-deoxygluconokinase [EC: 2.7.1.92] (inferred from 99% identity to pba:PSEBR_a3356)

Predicted SEED Role

"5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain" in subsystem Inositol catabolism (EC 2.7.1.92)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.92 or 4.1.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YUP5 at UniProt or InterPro

Protein Sequence (645 amino acids)

>Pf6N2E2_512 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (Pseudomonas fluorescens FW300-N2E2)
MGQTRFASGRQLDVICLGRLGVDLYAQQVGARLEDVASFAKYLGGSSANIAFGTARLGLR
SAMLSRVGDDHMGRFLVESLAREGCDVSGIKVDPERLTAMVLLGIKDRETFPLVFYRENC
ADMALRVEDIDETFIASSKALLITGTHFSTDGVYKASTQALDYAEKHNVKCVLDIDYRPV
LWGLAGKADGETRFVADQKVSQHVQGILPRFDLIVGTEEEFLIAGGSEDLLSALRTVRSL
TAATLVVKLGAQGCTVIHGAIPARLEDGALYPGVRVEVLNVLGAGDAFMSGFLAGWLEDA
SDERCCQLANACGGLVVSRHACAPAMPTRAELDYLFKSPVPITRPDQDAVLQRLHQVSVP
RKTWKQLFIFAFDHRWQLVELAQKGGRDLNCIGDLKQLFIQAVERVEADLQRQGIEADVG
LLADQRFGQDSLNAATGRGWWVARPVEVQNSRPLAFEHGRSIGSNLIAWPQEQIIKCLVQ
FHPDDEPLLRLEQEAQLMGLYKASQVSGHELLLEVIPPKDHPSPHPDVLYRALKRLYNLG
IFPAWWKIEAQSAQEWKQLDELIQQRDPYCRGVVLLGLNAPAAALAEGFRQASQSTTCRG
FAVGRTIFQEPSRAWLAGEIDDETLIRQVQGRFVELIEAWRSARA