Protein Info for Pf6N2E2_5103 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 10 to 238 (229 residues), 163.8 bits, E=5.9e-52
• TIGR01027: glutamate 5-kinase
  • amino acids 10 to 370 (361 residues), 433.8 bits, E=2.8e-134
• PF01472: PUA
  • amino acids 281 to 351 (71 residues), 73.6 bits, E=1e-24

Best Hits

Swiss-Prot: 98% identical to PROB_PSEPF: Glutamate 5-kinase (proB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 100% identity to pba:PSEBR_a4862)

MetaCyc: 44% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Sequence Analysis Tools

See A0A160A302 at UniProt or InterPro

Protein Sequence (372 amino acids)

>Pf6N2E2_5103 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA (Pseudomonas fluorescens FW300-N2E2)
MRSKVTGAQRWVVKIGSALLTADGKGLDRQAMAVWVEQMVALHEAGVELVLVSSGAVAAG
MSRLGWTSRPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLN
ARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFD
ADPRNNPDAKLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVGG
RLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDAGAVVALSQGNKSL
LPVGVKLVQGSFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMAE
PELVHRDNLILV